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| Year : 1984 | Volume
: 2
| Issue : 1 | Page : 1-6 |
Essential Regions Of The Lipopolysaccharide Of Pseudomonas Aeruginosa Responsible For Ito Biological Activities
Yuzuru J Homma
Correspondence Address:
Yuzuru J Homma
 Source of Support: None, Conflict of Interest: None  | Check |
Investigation of the relationship between the biological activities of the endotoxin of P. aeruginosa and chemical structure resulted in the dissociation of some of the biological activities of the endotoxin. Free lipid A was demonstrated to be responsible for the interferon-inducing activity, pyrogenicity and adjuvanticity. For manifesting inhibition of experimental tumor development or activation of proclotting enzyme of horseshoe crab (Tachypleus-tridentus), lipid A alone is not sufficient and a saccharide portion such as 3-deoxy-D-Manno-octulosonic acid is necessary to link the lipid A portion. That the polysaccharide portion plays a role in the activity of the complete lipopolysaccharide was also supported by the fact that chemical modification on the saccharide reduced the two activities drastically. Antitumor and interferon-inducing activities, adjuvanticity and activation of proclotting enzyme diminished by complete de-O-acylation by NH2 OH2 whereas interferon-inducing activity remained unchanged. Incomplete lipid A containing only amidelinked fatty acid is sufficient to induce interferon in vitro. None of the activities mentioned here was observed with degraded polysaccharide obtained after extraction of lipid A.
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