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Figure 2: Gel electrophoresis of 18S rDNA loop-mediated isothermal amplification assay for Acanthmoeba.(a) Lane M: molecular ladder; Lane 1: negative control (no DNA); Lanes 2, 3, 4, 5, 6, 7: results of loop-mediated isothermal amplification assay for DNA concentrations 1 ng, 100 pg, 10 pg, 1 pg, 100 fg, and 10 fg, respectively. (b) Lane M: molecular ladder; Lanes 1, 2, 3: corneal scraping samples positive for Acanthmoeba DNA; Lane 4: Plasmodium falciparum DNA; Lane 5: Toxoplasma gondii DNA; Lane 6: negative control (clinical sample with aetiology other than Acanthamoeba); Lane 7: negative control (no DNA).

Figure 2: Gel electrophoresis of <i>18S rDNA</i> loop-mediated isothermal amplification assay for <i>Acanthmoeba.</i>(a) Lane M: molecular ladder; Lane 1: negative control (no DNA); Lanes 2, 3, 4, 5, 6, 7: results of loop-mediated isothermal amplification assay for DNA concentrations 1 ng, 100 pg, 10 pg, 1 pg, 100 fg, and 10 fg, respectively. (b) Lane M: molecular ladder; Lanes 1, 2, 3: corneal scraping samples positive for <i>Acanthmoeba</i> DNA; Lane 4: <i>Plasmodium falciparum</i> DNA; Lane 5: <i>Toxoplasma gondii</i> DNA; Lane 6: negative control (clinical sample with aetiology other than <i>Acanthamoeba</i>); Lane 7: negative control (no DNA).