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Figure 4: Binding affinity of anti-H7 HA monoclonal antibodies with full-length H7N9 A/Anhui/1/2013 recombinant haemagglutinin determined by biolayer interferometry using an Octet RED96 instrument. The His6-tagged ligand (H7 A/Anhui/1/2013 recombinant haemagglutinin) in Tween-20 in phosphate-buffered saline was captured on Ni-NTA biosensors. A concentration series (trace 1-4: 200, 100, 50 and 25 nM) of the monoclonal antibodies was used to determine the binding affinity with the captured ligand; (a) MA-20 (b) MA-24 (c) MA-26 (d) MA-34 and (e) MA-36. The kinetic parameters were obtained by fitting the data to a 1:1 Langmuir model. The data points are represented by solid circles, and the fits are shown by dashed lines

Figure 4: Binding affinity of anti-H7 HA monoclonal antibodies with full-length H7N9 A/Anhui/1/2013 recombinant haemagglutinin determined by biolayer interferometry using an Octet RED96 instrument. The His<sub>6</sub>-tagged ligand (H7 A/Anhui/1/2013 recombinant haemagglutinin) in Tween-20 in phosphate-buffered saline was captured on Ni-NTA biosensors. A concentration series (trace 1-4: 200, 100, 50 and 25 nM) of the monoclonal antibodies was used to determine the binding affinity with the captured ligand; (a) MA-20 (b) MA-24 (c) MA-26 (d) MA-34 and (e) MA-36. The kinetic parameters were obtained by fitting the data to a 1:1 Langmuir model. The data points are represented by solid circles, and the fits are shown by dashed lines