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Figure 1: Reactivity in enzyme-linked immunosorbent assay of monoclonal antibodies generated against H7N9 A/Anhui/1/2013 recombinant haemagglutinin. Enzyme-linked immunosorbent assay plates were coated with recombinant haemagglutinin protein (200 ng/well) corresponding to H7N9 (A/Anhui/1/2013) and ovalbumin (200 ng/ml) as negative control, and varying concentrations of protein-G purified monoclonal antibodies (MA-20, -24, -26, -34, -36) were used to determine their reactivity as described in Materials and Methods section. The Y-axis represents mean ± standard error of the absorbance at 450 nm observed with the respective antibody tested in triplicate at a given concentration minus the absorbance obtained with ovalbumin

Figure 1: Reactivity in enzyme-linked immunosorbent assay of monoclonal antibodies generated against H7N9 A/Anhui/1/2013 recombinant haemagglutinin. Enzyme-linked immunosorbent assay plates were coated with recombinant haemagglutinin protein (200 ng/well) corresponding to H7N9 (A/Anhui/1/2013) and ovalbumin (200 ng/ml) as negative control, and varying concentrations of protein-G purified monoclonal antibodies (MA-20, -24, -26, -34, -36) were used to determine their reactivity as described in Materials and Methods section. The Y-axis represents mean ± standard error of the absorbance at 450 nm observed with the respective antibody tested in triplicate at a given concentration minus the absorbance obtained with ovalbumin