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Figure 1: Qualitative demonstration of siRNA transfection using BLOCK-iT™ following centrifugation enhanced transfection method. The images show the presence of siRNA (apple green) in the cytoplasm of the transfected cells. The nucleus of the cells is stained with propidium iodide and appears red. (a) luorescent microscopic picture of BLOCK-iT™ Oligos (scrambled siRNA) tranfected PBMCs counterstained with PI under ×10, excited at 536 nm showing nuclear component of the cells (b) The same region under ×10 magnifi cation, excited at 494 nm showing the presence of scrambled siRNA. (c) ×40 magnifi cation of transfected PBMC excited at 536 nm clearly showing the counter stain taken up by the nucleic acid and giving a red fl uorescence. (d) The same region excited at 494 nm showing the presence of transfected siRNA in the cytoplasm. (e, f) Cytoplasmic apple green fl uorescence of tranfected BLOCK-iT™ Oligos at ×20 and ×40, excited at 494 nm without propidium iodide counter staining

Figure 1: Qualitative demonstration of siRNA transfection using BLOCK-iT™ following centrifugation enhanced transfection method. The images show the presence of siRNA (apple green) in the cytoplasm of the transfected cells. The nucleus of the cells is stained with propidium iodide and appears red. (a)  luorescent microscopic picture of BLOCK-iT™ Oligos (scrambled siRNA) tranfected PBMCs counterstained with PI under ×10, excited at 536 nm showing nuclear component of the cells (b) The same region under ×10 magnifi cation, excited at 494 nm showing the presence of scrambled siRNA. (c) ×40 magnifi cation of transfected PBMC excited at 536 nm clearly showing the counter stain taken up by the nucleic acid and giving a red fl uorescence. (d) The same region excited at 494 nm showing the presence of transfected siRNA in the cytoplasm. (e, f) Cytoplasmic apple green fl uorescence of tranfected BLOCK-iT™ Oligos at ×20 and ×40, excited at 494 nm without propidium iodide counter staining