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Figure 1: Absorbance at 570 nm of PBMCs plated at a cell gradient of 1×103-2×106 cells/well. Y-axis – O.D values and X-axis – Number of cells plated were plotted. Cell concentration for plating is optimised to 2×104 as the next higher concentration of 1×105 showed considerably higher difference in O.D

Figure 1: Absorbance at 570 nm of PBMCs plated at a cell gradient of 1×10<sup>3</sup>-2×10<sup>6</sup> cells/well. Y-axis – O.D values and X-axis – Number of cells plated were plotted. Cell concentration for plating is optimised to 2×10<sup>4</sup> as the next higher concentration of 1×10<sup>5</sup> showed considerably higher difference in O.D