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Figure 2: Evaluation of DT fractionation. Treatment of DT with reducing sample buffer only (lane 1), DTT (lane 2), or digestion by trypsin (lane 3) resulting in partial fractionation. On the other hand, addition of trypsin leads to complete fractionation with increased MW of fractions over time (lanes 4-6)

Figure 2: Evaluation of DT fractionation. Treatment of DT with reducing sample buffer only (lane 1), DTT (lane 2), or digestion by trypsin (lane 3) resulting in partial fractionation. On the other hand, addition of trypsin leads to complete fractionation with increased MW of fractions over time (lanes 4-6)