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Figure 2: (a) SDS-PAGE analysis of expression of recombinant CTB in E. coli BL21 (DE3) using pQE_ctxB as expression vector. Lanes: 1, BL21 (DE3) containing pQE_ctxB before induction with IPTG; 2-6, in dicate 1-5 h after induction, respectively; 7, commercial CT (cholera toxin) used as positive control; 8 and 9, BL21 (DE3) containing pQE used as negative control. (b) Western blot analysis of heated samples probed with anti-CT antibody. Lanes: 1, expression of rCTB in BL21 (DE3) containing pQE_ctxB plasmid before induction with IPTG; 2-6, indicate 1-5 h after induction, respectively; 7, commercial CT used as positive control. (c) SDS-PAGE analysis of expression of recombinant CTB in Escherichia coli BL21 (DE3) using pAE_ctxB as expression vector. Lanes: 1, BL21 (DE3) containing pAE plasmid as the negative control; 2, protein size marker; 3, BL21 (DE3) containing pAE_ctxB before induction with IPTG; 4-8, indicate 1-5 h after induction, respectively. (d) Western blot analysis of heated samples probed with anti-CT antibody. Lanes: 1, expression of rCTB in BL21 (DE3) containing pAE_ctxB plasmid before induction with IPTG; 2-6, indicate 1-5 h after induction, respectively

Figure 2: (a) SDS-PAGE analysis of expression of recombinant CTB in <i>E. coli</i> BL21 (DE3) using pQE_<i>ctx</i>B as expression vector. Lanes: 1, BL21 (DE3) containing pQE_<i>ctx</i>B before induction with IPTG; 2-6, in dicate 1-5 h after induction, respectively; 7, commercial CT (cholera toxin) used as positive control; 8 and 9, BL21 (DE3) containing pQE used as negative control. (b) Western blot analysis of heated samples probed with anti-CT antibody. Lanes: 1, expression of rCTB in BL21 (DE3) containing pQE_<i>ctx</i>B plasmid before induction with IPTG; 2-6, indicate 1-5 h after induction, respectively; 7, commercial CT used as positive control. (c) SDS-PAGE analysis of expression of recombinant CTB in <i>Escherichia coli</i> BL21 (DE3) using pAE_<i>ctx</i>B as expression vector. Lanes: 1, BL21 (DE3) containing pAE plasmid as the negative control; 2, protein size marker; 3, BL21 (DE3) containing pAE_<i>ctx</i>B before induction with IPTG; 4-8, indicate 1-5 h after induction, respectively. <b>(</b>d<b>)</b> Western blot analysis of heated samples probed with anti-CT antibody. Lanes: 1, expression of rCTB in BL21 (DE3) containing pAE_<i>ctx</i>B plasmid before induction with IPTG; 2-6, indicate 1-5 h after induction, respectively