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Figure 1: (a) Plasmid extraction of Escherichia coli Top10F' cells transformed with pGEM-T_ctxB construct. Lanes: 1, pGEM_ctxB digested with EcoRI which shows ctxB fragment at 390 bp; 2, undigested pGEM_ctxB plasmid; 3, 1kb DNA size marker. (b) Enzymatic double digestion of pQE and pGEM_ctxB. Lanes: 1, pQE vector double digested with XhoI and NcoI; 3, pGEM_ ctxB double digested with XhoI and BspHI; 2, 1kb DNA size marker; 4, 100 bp DNA size marker. (c) Double digested pQE and pQE_ctxB plasmids. Lanes: 2, pQE vector double digested with XbaI and XhoI which shows fragment at 400 bp; 3, pQE_ctxB vector shows an 800 bp fragment containing ctxB gene; 1, 100 bp DNA size marker; 4, 1 kb DNA size marker. (d) Enzymatic double digestion of pAE_ctxB. Lanes: 2, undigested pAE_ctxB plasmid; 3 and 4, double digested pAE_ctxB with BamHI and HindIII, which shows ctxB fragment at 360 bp; 1, 1 kb DNA marker

Figure 1: (a) Plasmid extraction of <i>Escherichia coli</i> Top10F' cells transformed with pGEM-T_<i>ctx</i>B construct. Lanes: 1, pGEM_<i>ctx</i>B digested with <i>EcoR</i>I which shows <i>ctx</i>B fragment at 390 bp; 2, undigested pGEM_<i>ctx</i>B plasmid; 3, 1kb DNA size marker. <b>(</b>b<b>)</b> Enzymatic double digestion of pQE and pGEM_<i>ctx</i>B. Lanes: 1, pQE vector double digested with <i>Xho</i>I and <i>Nco</i>I; 3, pGEM_ <i>ctx</i>B double digested with <i>Xho</i>I and <i>Bsp</i>HI; 2, 1kb DNA size marker; 4, 100 bp DNA size marker. <b>(</b>c<b>)</b> Double digested pQE and pQE_<i>ctx</i>B plasmids. Lanes: 2, pQE vector double digested with <i>Xba</i>I and <i>Xho</i>I which shows fragment at 400 bp; 3, pQE_<i>ctx</i>B vector shows an 800 bp fragment containing <i>ctx</i>B gene; 1, 100 bp DNA size marker; 4, 1 kb DNA size marker. (d) Enzymatic double digestion of pAE_<i>ctx</i>B. Lanes: 2, undigested pAE_<i>ctx</i>B plasmid; 3 and 4, double digested pAE_<i>ctx</i>B with <i>Bam</i>HI and <i>Hind</i>III, which shows <i>ctx</i>B fragment at 360 bp; 1, 1 kb DNA marker