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Figure 2: Autophagic vesicles visualized with MDC fluorescent staining. Autophagic vesicles presented in the cytoplasm of WT-MEFs and RAPA-treated WT-MEFs infected by ST10 at early stage (1 h) of infection (a). There were no autophagic vesicles in WT-MEFs infected by ST8 (b) and SR-11(c) at early time points of infection. When WT-MEFs were treated with RAPA, autophagic vesicles occurred in the cells infected by ST10 (d), ST8 (e) and SR-11(f) at early stage. Autophagic vesicles were not observed in Atg5-/-MEFs infected by any bacteria (g, h, and i). Scale bar=25 μm

Figure 2: Autophagic vesicles visualized with MDC fluorescent staining. Autophagic vesicles presented in the cytoplasm of WT-MEFs and RAPA-treated WT-MEFs infected by ST<sub>10</sub> at early stage (1 h) of infection (a). There were no autophagic vesicles in WT-MEFs infected by ST<sub>8</sub> (b) and SR-11(c) at early time points of infection. When WT-MEFs were treated with RAPA, autophagic vesicles occurred in the cells infected by ST<sub>10</sub> (d), ST<sub>8</sub> (e) and SR-11(f) at early stage. Autophagic vesicles were not observed in Atg5-/-MEFs infected by any bacteria (g, h, and i). Scale bar=25 μm