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Figure 3: Ethidium bromide-stained agarose gel of PCR amplified products from extracted E. coli DNA amplified with primers EAF F and EAF R. Lane (L), DNA molecular size marker(100-bp ladder). Lane (E3), E. coli O125: K70(B15) show positive results withEAF gene. Lane (E5), E. coli O125: K70(B15) show positive results withEAF gene. Lanes (E1, 2, 4, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18 and 19) EPEC isolates show negative results withEAF gene. Lane (C), negative control

Figure 3: Ethidium bromide-stained agarose gel of PCR amplified products from extracted <i>E</i>. <i>coli</i> DNA amplified with primers <i>EAF</i> F <i>and EAF</i> R. Lane (L), DNA molecular size marker(100-bp ladder). Lane (E3), <i>E</i>. <i>coli</i> O125: K70(B15) show positive results withEAF gene. Lane (E5), <i>E</i>. <i>coli</i> O125: K70(B15) show positive results withEAF gene. Lanes (E1, 2, 4, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18 and 19) EPEC isolates show negative results withEAF gene. Lane (C), negative control