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Figure 2: In vitro synergy testing between meropenem and colistin for carbapenem resistant Klebsiella pneumoniae using the fi xed ratio method (E-test method 1) (a), (b) Determine MICs of mero and colistin individually (4μg / ml and 0.38μg / ml) (c) Place mero E-test strip(d) Mark on the agar surface coinciding with the MIC value of mero,cubate for one hour (e) Place colistin E-test strip after removing mero strip. MIC value of colistin coinciding with the mark (f) Read MIC after 18 hours, >2 fold reduction (0.012μg / ml) compared to colistin alone suggests synergy

Figure 2: In vitro synergy testing between meropenem and colistin for carbapenem resistant Klebsiella pneumoniae using the fi xed ratio method (E-test method 1) (a), (b) Determine MICs of mero and colistin individually (4μg / ml and 0.38μg / ml) (c) Place mero E-test strip(d) Mark on the agar surface coinciding with the MIC value of mero,cubate for one hour (e) Place colistin E-test strip after removing mero strip. MIC value of colistin coinciding with the mark (f) Read MIC after 18 hours, >2 fold reduction (0.012μg / ml) compared to colistin alone suggests synergy