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Figure 3 :The expression and purification of VP1 in pET21a vector. I = induced, UI = un-induced, M = protein molecular weight marker, UP = unpurified, P = purified. SDS-PAGE analyses of crude extracts of bacterial cultures both induced and un-induced (panel to the left of the molecular weight marker, M) and SDS-PAGE analysis of purified and un-purified VP1 (panel to the right of the molecular weight marker, M). Bands marked in the panel are VP1; the right-hand side band is the unpurified VP1 expressed in E coli and the left-hand side band is purified VP1. Arrows show desired bands of VP1

Figure 3 :The expression and purification of VP1 in pET21a vector. I = induced, UI = un-induced, M = protein molecular weight marker, UP = unpurified, P = purified. SDS-PAGE analyses of crude extracts of bacterial cultures both induced and un-induced (panel to the left of the molecular weight marker, M) and SDS-PAGE analysis of purified and un-purified VP1 (panel to the right of the molecular weight marker, M). Bands marked in the panel are VP1; the right-hand side band is the unpurified VP1 expressed in E coli and the left-hand side band is purified VP1. Arrows show desired bands of VP1