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Figure 2 :Separation of 600 bp V3 region of 16S rDNA gene of leptospiral serovars on Denaturant Gradient Gel electrophoresis under increasingly denaturing physical environment and at 600C. DNA, depending on the nucleotide sequence in the melted region, stops at different positions in the gel. Lane 1: L.interrogans, Pomona, serovar Pomona, Lane 2: L.biflexa, Semaranga, serovar Patoc, Lane 3: L.interrogans, Autumnalis, serovar rachmati

Figure 2 :Separation of 600 bp V3 region of 16S rDNA gene of leptospiral serovars on Denaturant Gradient Gel electrophoresis under increasingly denaturing physical environment and at 600C. DNA, depending on the nucleotide sequence in the melted region, stops at different positions in the gel. Lane 1: L.interrogans, Pomona, serovar Pomona, Lane 2: L.biflexa, Semaranga, serovar Patoc, Lane 3: L.interrogans, Autumnalis, serovar rachmati