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Figure 2: Results of polymerase chain reaction (PCR) (lane 1, a band of about 520 bp) and PCR-restriction fragment length polymorphism with ApaLI (lane 2, two fragments of about 350 bp and 170 bp) using the DNA sample isolated from the eggs from one patient. The same results were obtained when we used DNA samples isolated from the eggs of another patient or those from the metacercariae. Hundred basepair DNA ladders (Invitogen) were used to estimate the sizes of the bands (lane M)

Figure 2: Results of polymerase chain reaction (PCR) (lane 1, a band of about 520 bp) and PCR-restriction fragment length polymorphism with ApaLI (lane 2, two fragments of about 350 bp and 170 bp) using the DNA sample isolated from the eggs from one patient. The same results were obtained when we used DNA samples isolated from the eggs of another patient or those from the metacercariae. Hundred basepair DNA ladders (Invitogen) were used to estimate the sizes of the bands (lane M)