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Figure 1: (A) PERV proviral PCR for untreated tissues (using gag gene): Lane M represents the molecular marker (8-300bp). Lane N represents the negative control of human peripheral blood lymphocytes (PBL). Lane P represents the positive DNA control from porcine PK-15 cell lysates (Cell line in which PERV virus is released). Lanes 1 to 5 represent the porcine tissue samples positive for PERV virus resulted in amplification product of 187 bp. (B) PERV proviral PCR for decellularized tissues (using gag gene): Lane M represents the molecular marker (15 bp). Lane N represents the negative control of human PBL. Lane P represents the positive DNA control from porcine PK-15 cell lysate. Lanes 1 to 5 represent the decellularised treated tissue samples positive for PERV virus resulting in amplification product of 187 bp

Figure 1: (A) PERV proviral PCR for untreated tissues (using gag gene): Lane M represents the molecular marker (8-300bp). Lane N represents the negative control of human peripheral blood lymphocytes (PBL). Lane P represents the positive DNA control from porcine PK-15 cell lysates (Cell line in which PERV virus is released). Lanes 1 to 5 represent the porcine tissue samples positive for PERV virus resulted in amplification
product of 187 bp. (B) PERV proviral PCR for decellularized tissues (using gag gene): Lane M represents the molecular marker (15 bp). Lane N represents the negative control of human PBL. Lane P represents the positive DNA control from porcine PK-15 cell lysate. Lanes 1 to 5 represent the decellularised treated tissue samples positive for PERV virus resulting in amplification product of 187 bp