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Supplemental Figure 1: Effect of spacer molecule: Comparison of the performance of the array of 15-mers without (A) and with (B) a 15-dT spacer. Spots were prepared from 60 M oligonucleotide solutions in 1X Telechem spotting solution. Line 1: spots of probe (full match); lines 2 and 3: spots of probe (single mismatch); line 3: Hybridization with Cy3 end-labeled PCR fragment was performed in UniHyb buffer at 42 C for 6 h. Labeled probe was used at a fi nal concentration of 75 nM Figure 1: Behavior of different spotting solutions on an aldehyde surface. Triplicate spotting of an ULb54 oligonucleotide in different spotting solutions and hybridization with a Cy3 end-labeled probe of 350 bp obtained from UL54 ORF of HCMV (This set of oligonucleotides has been used for other subsequent optimization procedures including humidity, hybridization solution and optimal target concentration). The composition of the different solutions is shown in Materials and Methods. The solution containing 3X SSC and 0.01% SDS gave comparable hybridization results as the commercial spotting solution

Supplemental Figure 1: Effect of spacer molecule: Comparison of the performance of the array of 15-mers without (A) and with (B) a 15-dT spacer. Spots were prepared from 60 M oligonucleotide solutions in 1X Telechem spotting solution. Line 1: spots of probe (full match); lines 2 and 3: spots of probe (single mismatch); line 3: Hybridization with Cy3 end-labeled PCR fragment was performed in UniHyb buffer at 42 C for 6 h. Labeled probe was used at a fi nal concentration of 75 nM 
Figure 1: Behavior of different spotting solutions on an aldehyde surface. Triplicate spotting of an ULb54 oligonucleotide in different spotting solutions and hybridization with a Cy3 end-labeled probe of 350 bp obtained from UL54 ORF of HCMV (This set of oligonucleotides has been used for other subsequent optimization procedures including humidity, hybridization solution and optimal target concentration). The composition of the different solutions is shown in Materials and Methods. The solution containing 3X SSC and 0.01% SDS gave comparable hybridization results as the commercial spotting solution