a. SDS-PAGE analysis of the OMPs of L. interrogans serovar Icterohemorrhagiae. Lane M- molecular weight marker, lanes 1 and 2- OMPs from iron- replete and iron-limited organisms. b. Immuno-reactivity of the OMPs with serum samples from human patients. Panels A-D: serum samples with MAT titre of 1:3200 (A and B) and 1:800 (C and D); Panels E and F represent control serum samples. The arrows in Fig. 1a and 1b represent bands of 50, 55, 60 and 64 kDa respectively (64 kDa in Fig. 1B, Panel A is shown as*).