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   1992| January-March  | Volume 10 | Issue 1  
    Online since November 3, 2009

 
 
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Medical Microbiology For Students
Betty G Hobbs, Usha Gupta, Russel A William, S.P Thyagarajan
January-March 1992, 10(1):60-61
Full text not available  [PDF]
  930 52 -
C-Reactive Protein In Neonatal Sepsis
N Lakshmi, A Gururaj Kumar
January-March 1992, 10(1):29-31
C-reactive protein (CRP) was estimated in 75 cases of clinically diagnosed neonatal infections and the results were compared with bacterial cultures. CRP test was positive in 77.33% cases whereas bacterial cultures were positive in 58.66% cases. A positive CRP test was observed in 14 cases where the bacterial culture was negative, CRP test was highly sensitive, specific and reliable in cases of septicaemia but less reliable in cases of localized infection. CRP was not detected in 20 healthy neonates.
[ABSTRACT]   Full text not available  [PDF]
  748 215 -
Development Of A Simple Burn Mouse Model And Its Evaluation To Study The Efficacy Of Soframycin Against Pseudomonas Aeruginosa Infected Burns
Rauf Latif, S.P Thyagarajan, S Subramanian, S Ananthan, G Sridhar, K Udayasankar, V Arunagirinathan
January-March 1992, 10(1):23-28
To study burns in animal model, we report here construction of a simple modified burning device, which produces standard scald burns in mice or rats. The injury is inflicted by immersion of the area to be burned in boiling water while the animal is held in a protective template, which limits the area of exposure. Our study using this device has shown that large number of animals can be burned in a reasonably short time. Further work on the efficacy of soframycin as a topical applicant against Pseudomonas aeruginosa in such a model has shown that the infected but treated (G-III) group of mice showed a low mortality of only 8.5% when compared to the mortality of 66% seen in the untreated but infected group (Gll). Our findings of death in the control group (Gl) certainly warrants study on the role of other organisms in burns. This device seem to be an ideal choice to study the efficacy of topical antibiotics and burn metabolisms in animals.
[ABSTRACT]   Full text not available  [PDF]
  807 56 -
Primary cytomegalovirus Infection In Pregnant and Non-Pregnant Women In India
Arti Kapil, Shobha Broor
January-March 1992, 10(1):53-55
Primary cytomegalovirus (CMV) infection in pregnant women can lead to fatal or symptomatic infections in the foetus. In the present study we have shown evidence of primary CMV infection in pregnancy by demonstrating the presence of CMV specific lgM antibodies. Out of 31 pregnant women, 4(12.9%) had evidence of primary CMV infection, whereas in the women who had given birth to children suffering from symptomatic congenital infections, evidence of primary CMV infection was seen in 3 out of 23 (13%) women. In a heterogeneous population of women in child bearing age, 12/80 (15%) were found to be seronegative for CMV specific lgG antibodies suggesting their susceptibility to acquire a primary CMV infection in pregnancy.
[ABSTRACT]   Full text not available  [PDF]
  610 91 -
Evaluation Of Rhelax ASO Slide Test For Antistreptolysin '0'
A.K Kapoor, R Gupta, K Prakash
January-March 1992, 10(1):32-34
In present study 120 serum samples obtained from sore throat, rheumatic fever & rheumatic heart disease were screened with Rhelax ASO slide test for antistreptolysin ‘O’ and by conventional method. It was observed that results were same in 93.3%, 4.0% sera gave false positive tests and 2.5% sera showed false negative tests, with Rhelax ASO slide test.
[ABSTRACT]   Full text not available  [PDF]
  574 85 -
Incidence Of Nocardiosis In Bronchopulmonary Diseases
Amarjit Kaur Gill, Shashi Kanta, Arun Oberoi, S.K Gupta
January-March 1992, 10(1):45-47
Nocardiosis is a systemic disease, which has special predilection for lungs. The pulmonary nocardiosis closely mimic tuberculosis clinically and radio logically 1,2. Occasionally by haematogenous spread it may extend to various organs more particularly to brain causing brain abscess. Nocardia Spp. are soil borne saprophytes3, 4. The present study is undertaken to find out the incidence of pulmonary nocardiosis in various bronchopulmonary disorders in Patiala area.
[ABSTRACT]   Full text not available  [PDF]
  532 54 -
Invasion Of Paranasal Sinuses By Aspergillus Fumigatus : A Case Report
Arti Kapil, Uma Banerjee, Subarinder Kumar
January-March 1992, 10(1):42-44
A case of aspergillosis of the ethomoidal sinuses extending to the orbit is reported. The patient [resented with a history of nasal obstruction, headache and gradual shrinkage in the size of the left eyeball. On examination, the left nasal cavity showed a reddish – white mass obstructing the nasal cavity. CT scan showed a soft tissue mass in the left nasal cavity, ethmoids and forntal sinuses. Direct examination of the excised tissue revealed the presence of septate, branching fungal elements. A. fumigatus was repeatedly isolated in culture. The patient was subjected to surgery followed by treatment with antibacterial and anti-fungal antibiotics.
[ABSTRACT]   Full text not available  [PDF]
  526 49 -
Association Of C.Trachomatis, G.Vaginalis And Mycoplasma With Intrauterine Contraceptive Device Use
Meera Sharma, Indu Gupta, N Nayak, P.R Handa
January-March 1992, 10(1):48-52
Genital microbiological flora (Chlamydia trachomatis, Mycoplasma hominis, Ureaplasma ureaiyticum and Gardnerella vaginalis) was compared in IUCD users and non-users. Out of a total of 104 subjects studied, 16 IUCD users and 26 nonusers underwent voluntary tubectomy and hence tubal segments were also studied. No statistically significant difference in the isolation rates of genital mycoplasmas and G. varinalis was observed in the vaginal and cervical swabs of users and non-users. Tubal samples from 16 IUCD users and 26 nonusers did not show any significant difference in the detection rates of C.trachomatis antigen. In none of the tubal segments, G. varinalis and Mycoplasmas could be isolated. This study, thus, showed that IUCD use is devoid of risk of infection in addition to being an effective method of temporary contraception.
[ABSTRACT]   Full text not available  [PDF]
  497 55 -
Comparison Of Latex Agglutination Test With Elisa And Electron Microscopy For Detection Of Human Rotavirus In Stool
N.T Gokhale, T Reddeppa, A.N Sarma, G.R Bhangui
January-March 1992, 10(1):56-59
A Commercial latex agglutination (LA) test was compared with ELISA and electron microscopy (EM) for detection of rotavirus antigen in 100 stool specimens of children with acute diarrhoea. Eighty-five stool specimens (85%) were either positive or negative with all the three tests used while 15 specimens (15%) gave contradictory results. Only 1 specimen was positive by LA test but not the ELISA or EM. The Sensitivity of LA test was 45.8 percent (11 of 24), and that of EM was 98.6 percent (75 of 76), and that of EM was 100 percent (76 of 76). The ELISA test detected more positive (24) specimens than the EM (14) or the LA test (12). The sensitivity of LA test was found to be low, but it is highly specific and rapid, hence may be useful in certain situations.
[ABSTRACT]   Full text not available  [PDF]
  484 59 -
Human Papilloma Virus In Normal, Preneoplastic And Neoplastic Epithelia Of The Cervix Uteri
K Udayasankar, M Elanchezhiyan, S.P Thyagarajan, B.C Das, R Suseela, S Subramanian
January-March 1992, 10(1):16-22
Identification of Human papilloma virus infection of the genital tract was made on patients attending the Gynaecology O.P.. Cytomorphology and Southern blot hybridization were compared for their ability to detect HPV. Separate cervico vaginal smears from 100 patients were subjected to Pap smear study. Concurrent punch biopsies from fifty patients and 12 controls were tested for HPV types 6/11, 16, 18, and 16/18 by Southern blot hybridization. While 48.4% (30/62) of them were hybridization positive, 80.6% (50/62) showed positivity for HPV associated cytopathology. HPV 16 was found in all grades of cervical lesions (80%). HPV 6/11 was present in initial grades of cervical lesions and HPV 18 in higher grades of lesion. A correlation of 83% was observed between DNA hybridization and cytomorphology in the detection of cervical neoplasia.
[ABSTRACT]   Full text not available  [PDF]
  468 70 -
Current Status Of H. Influenzae Type B Vaccines
Mathuram Santosham
January-March 1992, 10(1):3-15
Haemophilus influenzae type b (Hib) is the leading cause of meningitis in children below 5 years of age. Majority of cases of Hib occur in infants below 2 years of age. Until recently the only vaccine available against this disease contained the pure polysaccharide (PRP) of (Hib-PRP Vaccine). The Hib-PRP vaccine was demonstrated to be efficacious in infants above 18 months of age but not below that age. This product was licensed for routine use in the U.S.A. for children aged 24 months or more. Recently a hyper immune globulin termed bacterial polysaccharide immune globulin (BPIG) was prepared by immunizing adult donors with Hib-PRP, meningococcal and pneumococcal vaccines. BPIG has been demonstrated to prevent Hib infections when it is administered to infants at 4-month intervals. However, BPIG has not been licensed for routine use in the U.S.A.A number of new Hib conjugate vaccines have also been developed in the last few years by covalently linking the Hib-PRP to different carrier proteins. Four different Hib conjugate vaccines have undergone clinical trials in the U.S.A. Two of these vaccines, HbOC (Hib capsular oligosaccharide linked to CRM 197) and Hib-OMPC (Hib capsular polysaccharide linked to Neisseria meningitides outer membrane protein complex) have been demonstrated to protect infants aged 2 months or more from Hib disease. Both HbOC and Hib-OMPC are currently licensed for routine use in the U.S.A. The widespread use of these vaccines should have a substantial impact in reducing morbidity and mortality from Hib disease.
[ABSTRACT]   Full text not available  [PDF]
  450 58 -
Primary Mycotic Keratitis Among Tea - Garden Workers
K.K Haldar, A.R Banerjee, A.K Chakraborthy, S Basak
January-March 1992, 10(1):38-41
Corneal scrapings from 203 clinically suspected cases of mycotic keratitis were investigated for evidence of fungal infection. In 54 cases both bacteria and fungi were found in smear examination and was correlated by culture. In 63 cases only fungal elements found in smear examination and single fungal spp. grown on culture. The incidence among males (55.5%) was little more than females (44.4%) with slight preponderance in the 4th decade age group. All the patients gave definite history of corneal trauma while working in the tea garden. Aspergillus was the commonest (50.8%) fungal spp. isolated followed by Candida spp. (17.5%), Acremonium spp. (12.7%), Pencillium spp. (6.3%), Curvularia spp. (4.8%), Fusarium spp. (3.2%) and Aureobasidium, Alternaria, Cladosporium spp. (1.6%) each.
[ABSTRACT]   Full text not available  [PDF]
  422 65 -
Isolation Of Thymine Dependent E.Coli From A Patient On Cotrimoxazole Therapy And Its Clinical Significance
B.M Hemashettar, Suman Hanchinamani, C.S Patil, Rajendra Nerli
January-March 1992, 10(1):35-37
Thymine dependent Escherichia Coli was isolated from the urine of a patient on Co-trimoxazole therapy. These mutants require exogenous thymine or thymidine for growth and form long filaments in media deficient in these substrates. As these strains are not affected by Co-trimoxazole therapy, it should be replaced by other antibacterial to which the isolate is susceptible.
[ABSTRACT]   Full text not available  [PDF]
  441 42 -
Hepatitis B Vaccination In India
S.P Thyagarajan
January-March 1992, 10(1):1-2
Full text not available  [PDF]
  387 54 -

2004 - Indian Journal of Medical Microbiology
Published by Wolters Kluwer - Medknow

Online since April 2001, new site since 1st August '04