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  Citation statistics : Table of Contents
   1990| April-June  | Volume 8 | Issue 2  
    Online since January 16, 2010

 
 
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Characterization Of Klebocin Plasmids In Klebsiella Pneumoniae
D. R Arora, T. D Chugh
April-June 1990, 8(2):36-39
Properties of kelbocin plasmids ie stability, curing phage restriction, pilus formation, fertility inhibition and molecular weight was studied in 6 strains of K.pneumoniae. These Plasmids were found to be highly stable in the host strains of K. pneumonia in which they persisted for more than 15 years. However, they were less stable in recipient strain of E. coli. Two of these strains lost these plasmids in 1.5 years while other 4 lost these in 2 years. Caring could not be accomplished with sub-inhibitory concentrations of acriflavine, acridine orange and sodium dodecyl sulphate in any of them. However, one strains could be cured by growing it at 44 + 0.5 C on third subculture. Molecular weight of klebocin plasmids has been found to be 10 X 106.
[ABSTRACT]   Full text not available   
  - 499 6
Evaluation Of HSV-2 Bgl-II N Fragment
J Kishore, N Manjunatha, P Seth
April-June 1990, 8(2):40-43
A recently developed sub-genomic DNA probe (7.2kb), HSV-2 Bgl-II N fragment cloned in PKC-7 plasmid has been evaluated in comparison to whole genomic HSV-2 DNA for specific detection of herpes simplex virus type-2 by filter hybridization. The probes were vector freed, nick-translated using 32pdCTP to a specific activity of 0.5-1 x 108 cpm/ug DNA. The whole gonomic HSV-2 DNA hybridized under stringent conditions with mock-infected cells and cells infected with other viruses as reveled by autoadiography. In contrast HSV-2 Bgl-II N fragment probe hybridized specifically only with HSV-2 infected vero cells and could detect 103 or more of such cells. Storage of the infected cells prior to hybridization at 4 C or –70 C for 24 hours did not alter the sensitivity or specificity of detection. Thus HSV-2 Bgl II N fragment can be used as rapid, specific and sensitive probe for the detection of herpes simplex virus type-2 infected cells.
[ABSTRACT]   Full text not available   
  - 446 4
E.Coli. Serotypes Isolated From Sites Other Than Urinary And Intestinal Region
L. N Rao Bahu, D Gowal, S Gupta, A. P Chaturvedi
April-June 1990, 8(2):44-47
Ninety eight E. coli strains isolated form sites other than intestinal and urinary tract were serotyped. The most common E.coli serotypes encountered were 017, 024, 068, 05, 020, 057, 0106, 0109 and 0132 in that order. Majority of vaginal infections were due to E. coli serotypes known as urinary pathogens, however none of these were observed in wound infections. Restricted group of E.coli serotypes was encounter which causes extra-intestinal and extra-urinary infections. Very high rate of antibiotic resistance (77.5%) was observed among these serotypes. Significance of these findings have been discussed.
[ABSTRACT]   Full text not available   
  - 467 5
A Minimal Synthetic Liquid Medium For Germ Tube Production By Candida Albicans
V Bhatia, H Joshi, K. R Joshi
April-June 1990, 8(2):48-52
A series of synthetic media consisting of various combinations of six amino acids (L-serine, DL-Methionine, L-ornithine, L-phenylalanine, L-alanine, L-leucine) in concentration of 20 mg% each, salt solution (containing potassium dihydrogen phosphate, calcium chloride, magnesium sulphate, ammonium sulphate), glucose and vitamin B-complex (Microbion forte) were evaluated for their ability to induce formation of germtubes in a strains of Candida albicans. The results were compared with that obtained in human serum. Formation of germtube occurred maximally (45.2%) in a simple synthetic medium consisting of glucose 100mg% and serine, ornithine and methionine 20 mg% each only. Compared to this in human serum the strain formed 38.5% germ tubes only. Subsequently 47 isolates of different species of Candida consisting of Candida albicans (30), Candida tropicalis (13), Candida pseudotropicalis (2), Candida Parapsilosis (2) were tested for formation of germ tubes in this simple synthetic medium and in serum. All the isolates of Candida albicans and 2 of Candida tropicalis which formed germ tubes in human serum also formed these in this medium, the number of germ tube formed however, was slightly more in the synthetic medium. The medium is thus equally specific and sensitive, and being simple and synthetic is recommended for use in germ tube test and in the study of factors involved and biochemical events occurring during the formation of germ tubes.
[ABSTRACT]   Full text not available   
  - 742 9
Immunological Reactivity Of Lungs : Immunoglobulin Secreting Cells In Pneumocystis Carinii Infected Rates.
H Vohra, N. K Ganguly, R. C Mahajan
April-June 1990, 8(2):53-59
Experimental pneumocystis carinii infection was induced in young rats by injecting hydrocortisone and feeding low protein diet. The number of immunoglobulin secreting cells were checked by reverse plaque assay in the bronchoalveolar lavage from infected animals weekly. It was seen that IgM and IgG secreting cells decreased in number even before P. carinii cysts were detected in the lavage. IgA secreting cells decreased in number till 5th week of infection and later increased in the experimentally infected animals. In the group of animals injected with steroids alone there was a steady decline in immunoglobulin secreting cells till the completion of study. The role of raised IgA levels during peak parasite load discussed.
[ABSTRACT]   Full text not available   
  - 444 5
“Rat Mite Dermatitis-A Diagnostic Enigma”
P. S Grover, M. L Gupta, N. L Sharma, R. C Sharma
April-June 1990, 8(2):60-61
An outbreak of ratmite dermatitis is presented. The causative agent was identified as ornithonyssus bacoti. The outbreak occurred in a laboratory. The source of the mite was traced and the mites were recovered from the surroundings of the laboratory. Other evidences of infestation of the laboratory by rats were also seen.
[ABSTRACT]   Full text not available   
  - 483 3
Bacteriological Study In Septic Abortion
M Singh, S. K Mathur, L Singh, U. C Chaturvedi
April-June 1990, 8(2):62-67
Sixty cases of septic abortion were subjected for clinical examination and bacteriological study. In this study most of cases showed polybacterial infections. Infection was found more severe or ended with complication, when there was mixture of anaerobic and aerobic bacteria.
[ABSTRACT]   Full text not available   
  - 585 7
Susceptibility Of Pseudomonas Aeruginosa To Newer Cephalosporins
B Mishra, R Kandahari, A Mandal
April-June 1990, 8(2):68-71
Seventy strains of pseudomonas aeruginosa isolated form clinical samples of hospital-infected cases were tested for sensitivity to cerftazidime, cefotaxime and cephazoline. Five strains (7%) were resistant to ceftazidime, 28 (40%) to cerfotaxime and 56 (80%) to cephazoline, MIC of ceftazidime for the resistant strains was 4.0 mg/L, whereas the sensitive strains were inhibited by 0.5-1.0 mg/L of the drug. Thirty four percent of the strains were betalacamase producers. In vitro cephalosporin resistance was compared to that of aminoglycosides viz. gentamicin (39%), sisomicin (57%) and tobramycin (45%).
[ABSTRACT]   Full text not available   
  - 525 13
Environmental Surveillance And Its Role In Post-Operative Hospital Acquired Infections
A Thakur, B Mishra, A Mandal
April-June 1990, 8(2):72-77
In a 250 bedded hospital in New Delhi environmental surveillance was carried out over a period of 1.5 years. Air in various O.T.’s, ICU and post operative ward was found unsatisfactory 60-80% times. From disinfectants and suction bottles pathogens were recovered 21.16% & 74.24% times respectively, commonest being Pseudomonas, Klebsiella and E, coli, 64.7% people were found to be nasal carriers of staphylococcus aureaus. (Post operative infection rate was 2.34% only. The commonest bacteria isolated were Pseudomonas, Klebsiella and Staph aureus).
[ABSTRACT]   Full text not available   
  - 527 6

2004 - Indian Journal of Medical Microbiology
Published by Wolters Kluwer - Medknow

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