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Year : 2019  |  Volume : 37  |  Issue : 3  |  Page : 337--344

Geographically distinct North-East Indian Helicobacter pylori strains are highly sensitive to clarithromycin but are levofloxacin resistant

Shweta Mahant1, Amresh Kumar Sharma2, Valentina Gehlot1, Asish Kumar Mukhopadhyay3, Ayushi Chhawchharia1, Sangitanjan Dutta4, Anil Agarwal4, Anup Som2, Kunal Das5, Rajashree Das1 
1 Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradesh, India
2 Centre of Bioinformatics, University of Allahabad, Allahabad, Uttar Pradesh, India
3 Division of Bacteriology, National Institute of Cholera and Enteric Diseases, Kolkata, West Bengal, India
4 Department of Gastroenterology, Gauhati Medical College, Guwahati, Assam, India
5 Department of Gastroenterology and Hepatology, Max Super Specialty Hospital, Vaishali, Ghaziabad, Uttar Pradesh, India

Correspondence Address:
Dr. Rajashree Das
Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradesh
India

Purpose: Helicobacter pylori causes various gastro-intestinal diseases. Antibiotic resistance to commonly used antibiotics for the treatment of H. pylori infection is the major cause for treatment failure. The aim of this study is to determine the antimicrobial susceptibility pattern for clarithromycin and levofloxacin and find the evolutionary relationship of the partial sequence of 23S rRNA and gyraseA gene of H. pylori by phylogenetic analysis. Materials and Methods: A total of 46 H. pylori strains were tested for clarithromycin and levofloxacin susceptibility pattern and phylogenetic tree were reconstructed by PhyML software. Results: In this study, we observed that only 6.5% of North-East Indian H. pylori strains were resistant for clarithromycin showing mutation at A2143G and T2182C positions of 23S rRNA gene. Resistance for levofloxacin was observed in 89.1% of the H. pylori strains showing mutations at asparagine to lysine at 87 and aspartic acid to glycine/tyrosine/asparagine at 91 positions of gyraseA gene. The phylogenetic tree of the partial sequence of 23S rRNA and gyraseA gene depicts that the North-East Indian strains falls in different cluster when compared to other countries. Conclusions: Resistance for clarithromycin was less in North-East Indian strains but high for levofloxacin indicating that first-line therapy may be best and effective for eradication of H. pylori in this region. This study is the first report that showed antibiotic susceptibility pattern for clarithromycin and levofloxacin by mutation analysis. By partial sequencing of 23s rRNA and gyraseA gene, we found that North-East Indian strains are geographically distinct.

How to cite this article:
Mahant S, Sharma AK, Gehlot V, Mukhopadhyay AK, Chhawchharia A, Dutta S, Agarwal A, Som A, Das K, Das R. Geographically distinct North-East Indian Helicobacter pylori strains are highly sensitive to clarithromycin but are levofloxacin resistant.Indian J Med Microbiol 2019;37:337-344

How to cite this URL:
Mahant S, Sharma AK, Gehlot V, Mukhopadhyay AK, Chhawchharia A, Dutta S, Agarwal A, Som A, Das K, Das R. Geographically distinct North-East Indian Helicobacter pylori strains are highly sensitive to clarithromycin but are levofloxacin resistant. Indian J Med Microbiol [serial online] 2019 [cited 2020 Aug 8 ];37:337-344
Available from: http://www.ijmm.org/article.asp?issn=0255-0857;year=2019;volume=37;issue=3;spage=337;epage=344;aulast=Mahant;type=0