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Year : 2018  |  Volume : 36  |  Issue : 4  |  Page : 537--540

Transcriptional response of AcrEF-TolC against fluoroquinolone and carbapenem in Escherichia coli of clinical origin

Shiela Chetri1, Anutee Dolley1, Deepshikha Bhowmik1, Debadatta Dhar Chanda2, Atanu Chakravarty2, Amitabha Bhattacharjee1 
1 Department of Microbiology, Assam University, Silchar, Assam, India
2 Department of Microbiology, Silchar Medical College and Hospital, Silchar, Assam, India

Correspondence Address:
Dr. Amitabha Bhattacharjee
Department of Microbiology, Assam University, Silchar, Assam
India

Introduction: Efflux pump systems constitute a major means of intrinsic resistance in Escherichia coli. AcrEF-TolC pump is known to exhibit higher expression level in quinolone resistant isolates. However, the transcriptional response of this pump is yet to be known when exposed to quinolone and other group of antibiotics. Objective: The present study analyses the transcriptional response of AcrEF-TolC in the presence of quinolones and carbapenems. Methodology: A total of 167 non-duplicate clinical isolates from Silchar medical college and Hospital, Silchar, India were included in this study. Of which 27 were devoid of any carbapenemase activity and among them 13 isolates showed overexpression of AcrE and AcrF gene. Transcriptional response of AcrE was directly proportional to increasing concentration of levofloxacin and ofloxacin. However, the response of AcrE and AcrF was inconsistent with carbapenems. Result: The study isolates showed susceptibility towards amikacin (68.4%), gentamicin (59.6%), cefepime (52.7%) and pipercillin/tazobactam (48.3%). The present investigation highlights that apart from qnr genes and mutational changes in gyr region, AcrEF-TolC plays a major role in fluoroquinolone resistance in this part of the world. Conclusion: Upregulation of AcrE in the presence of levofloxacin and ofloxacin warrants further investigation to establish their active role in efflux of this drug.

How to cite this article:
Chetri S, Dolley A, Bhowmik D, Chanda DD, Chakravarty A, Bhattacharjee A. Transcriptional response of AcrEF-TolC against fluoroquinolone and carbapenem in Escherichia coli of clinical origin.Indian J Med Microbiol 2018;36:537-540

How to cite this URL:
Chetri S, Dolley A, Bhowmik D, Chanda DD, Chakravarty A, Bhattacharjee A. Transcriptional response of AcrEF-TolC against fluoroquinolone and carbapenem in Escherichia coli of clinical origin. Indian J Med Microbiol [serial online] 2018 [cited 2019 Dec 10 ];36:537-540
Available from: http://www.ijmm.org/article.asp?issn=0255-0857;year=2018;volume=36;issue=4;spage=537;epage=540;aulast=Chetri;type=0