Indian Journal of Medical Microbiology Home 

ORIGINAL ARTICLE
[View FULLTEXT] [Download PDF]
Year : 2018  |  Volume : 36  |  Issue : 4  |  Page : 522--525

Occurrence of II and V staphylococcal cassette chromosome mec types among coagulase-negative staphylococci from Northeastern part of India

Deepshikha Bhowmik, Pinki Chakraborty, Rituparna Bhowmick, Rakesh Sarkar, Shiela Chetri, Amitabha Bhattacharjee 
 Department of Microbiology, Assam University, Silchar, Assam, India

Correspondence Address:
Dr. Amitabha Bhattacharjee
Department of Microbiology, Assam University, Silchar - 788 011, Assam
India

Introduction: Coagulase Negative Staphylococci, the most commonly isolated pathogen are becoming emerging threats to the community as well as to the nosocomial environment. The present study underscores the distribution of Staphylococcal cassette chromosome mec (SCCmec) types among Methicillin resistant Coagulase Negative Staphylococci from the environmental origin. Methods and Materials: Environmental and food sample (n = 460) from different location of northeastern region of India were collected for a period of one year and were phenotypically and genotypically screened using cefoxitin disc and PCR techniques for mecA and mecC gene detection. All the MR-CoNS isolates possessing mecA gene were subjected to 16srDNA sequencing for species identification. SCCmec typing was determined by evaluating using primer sets from type I to type V. Antibiotic susceptibility testing was performed for all the isolates. Statistical analysis with chi-square test using SPSS-21 statistical software. Results: Methicillin resistance shown by one hundred forty three isolates were carried out for molecular analysis, among them 53.84% serves as mecA carrier. Distribution of Staphylococcus haemolyticus was more frequent and was found that SCCmec types II and V were predominant among the study isolates. Linezolid was the drug of choice for the CoNS isolates. Statistical analysis showed an insignificant result for the tested antibiotics and SCCmec types. Conclusion: This study therefore interprets the relative importance of SCCmec types among MR-CoNS isolates.

How to cite this article:
Bhowmik D, Chakraborty P, Bhowmick R, Sarkar R, Chetri S, Bhattacharjee A. Occurrence of II and V staphylococcal cassette chromosome mec types among coagulase-negative staphylococci from Northeastern part of India.Indian J Med Microbiol 2018;36:522-525

How to cite this URL:
Bhowmik D, Chakraborty P, Bhowmick R, Sarkar R, Chetri S, Bhattacharjee A. Occurrence of II and V staphylococcal cassette chromosome mec types among coagulase-negative staphylococci from Northeastern part of India. Indian J Med Microbiol [serial online] 2018 [cited 2019 Aug 17 ];36:522-525
Available from: http://www.ijmm.org/article.asp?issn=0255-0857;year=2018;volume=36;issue=4;spage=522;epage=525;aulast=Bhowmik;type=0