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Year : 2017  |  Volume : 35  |  Issue : 1  |  Page : 33--36

Prevalence and characterization of rotaviruses in children hospitalized for diarrheal disease in a tertiary care hospital, Pune

Sae Satish Pol1, Ashwini Kacharu Dedwal1, Sujata Sudhir Ranshing2, Shobha Dattatray Chitambar2, Sushma Narayan Pednekar1, Renu Satish Bharadwaj1 
1 Department of Microbiology, B.J. Government Medical College, Sassoon General Hospital, Pune, Maharashtra, India
2 Rotavirus Division, National Institute of Virology, Pune, Maharashtra, India

Correspondence Address:
Sae Satish Pol
Department of Microbiology, B.J. Government Medical College, Sassoon Hospital Campus, Station Road, Pune - 411 001, Maharashtra
India

Background: Diarrhoea remains the second most common cause of death among children below 5 years globally. Among various enteric pathogens, rotavirus appears to be the most important aetiological agent of acute gastroenteritis in infants and young children. Increased understanding of epidemiology of rotavirus infections is needed to improve the vaccine efficacy. Aim: This study aims to determine prevalence rotavirus infection and prevalent circulating strains of rotavirus in and around Pune. Setting and Design: Prospective hospital-based study. The study was approved by Institutional Ethical Committee. Materials and Methods: Stool samples (n = 100) were collected from children aged <5 years, hospitalised for acute diarrhoea in paediatric ward at a tertiary care hospital. Samples were subjected for rotavirus antigen capture ELISA. The viral RNA was subjected to multiplex reverse transcription polymerase chain reaction to amplify VP7 genotypes G1–G4, G8–G10 and G12 and VP4 genotypes P[4], P[6], P[8], P[9], P[10] and P[11]. Nontypable rotavirus strains were sequenced. Results: About 35% stool samples were positive for rotavirus antigen by ELISA. G9P[4] (28.6%) was found to be the most prevalent rotavirus strain. The detection of emerging strain G12P[6] (14.3%) and rare reassortant strain G9P[4] was the significant finding. Conclusion: Genotypes found in circulation are not present in the currently used vaccine. Thus, an emergence of newer genotypes over a period calls for the continued surveillance and genomic characterisation of rotaviruses to improve the vaccine efficacy.

How to cite this article:
Pol SS, Dedwal AK, Ranshing SS, Chitambar SD, Pednekar SN, Bharadwaj RS. Prevalence and characterization of rotaviruses in children hospitalized for diarrheal disease in a tertiary care hospital, Pune.Indian J Med Microbiol 2017;35:33-36

How to cite this URL:
Pol SS, Dedwal AK, Ranshing SS, Chitambar SD, Pednekar SN, Bharadwaj RS. Prevalence and characterization of rotaviruses in children hospitalized for diarrheal disease in a tertiary care hospital, Pune. Indian J Med Microbiol [serial online] 2017 [cited 2019 Sep 23 ];35:33-36
Available from: http://www.ijmm.org/article.asp?issn=0255-0857;year=2017;volume=35;issue=1;spage=33;epage=36;aulast=Pol;type=0