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Year : 2016  |  Volume : 34  |  Issue : 3  |  Page : 350--352

Distribution of genes encoding aminoglycoside-modifying enzymes among clinical isolates of methicillin-resistant staphylococci

N Perumal, S Murugesan, P Krishnan 
 Department of Microbiology, Dr. ALM PG Institute of Basic Medical Sciences, University of Madras, Chennai, India

Correspondence Address:
P Krishnan
Department of Microbiology, Dr. ALM PG Institute of Basic Medical Sciences, University of Madras, Chennai
India

The objective of this study was to determine the distribution of genes encoding aminoglycoside-modifying enzymes (AMEs) and staphylococcal cassette chromosome mec (SCCmec) elements among clinical isolates of methicillin-resistant staphylococci (MRS). Antibiotic susceptibility test was done using Kirby-Bauer disk diffusion method. The presence of SCCmec types and AME genes, namely, aac (6«SQ»)-Ie-aph (2«SQ»«SQ»), aph (3«SQ»)-IIIa and ant (4«SQ»)-Ia was determined using two different multiplex polymerase chain reaction. The most encountered AME genes were aac (6′)-Ie-aph (2«SQ»«SQ») (55.4%) followed by aph (3«SQ»)-IIIa (32.3%) and ant (4«SQ»)-Ia gene (9%). SCCmec type I (34%) was predominant in this study. In conclusion, the aac (6«SQ»)-Ie-aph (2«SQ»«SQ») was the most common AME gene and SCCmec type I was most predominant among the MRS isolates.

How to cite this article:
Perumal N, Murugesan S, Krishnan P. Distribution of genes encoding aminoglycoside-modifying enzymes among clinical isolates of methicillin-resistant staphylococci.Indian J Med Microbiol 2016;34:350-352

How to cite this URL:
Perumal N, Murugesan S, Krishnan P. Distribution of genes encoding aminoglycoside-modifying enzymes among clinical isolates of methicillin-resistant staphylococci. Indian J Med Microbiol [serial online] 2016 [cited 2020 Sep 30 ];34:350-352
Available from: http://www.ijmm.org/article.asp?issn=0255-0857;year=2016;volume=34;issue=3;spage=350;epage=352;aulast=Perumal;type=0