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Year : 2015  |  Volume : 33  |  Issue : 5  |  Page : 143--148

Spontaneous clearance of chronic hepatitis C infection is associated with an internal ribosomal entry site IIId stem loop structure variant

NG Bader El Din1, MM El Hefnawy2, MH Omran1, RM Dawood1, Y El Abd1, MK Ibrahim1, MK El Awady1 
1 Department of Microbial Biotechnology , National Research Center, Tahrir, Dokki, Cairo, Egypt
2 Department of Informatics and Systems, National Research Center, Tahrir, Dokki, Cairo, Egypt

Correspondence Address:
N G Bader El Din
Department of Microbial Biotechnology , National Research Center, Tahrir, Dokki, Cairo
Egypt

Aim: To investigate if any mutations in hepatitis C virus (HCV) internal ribosome entry site (IRES) can inhibit the translation of viral polyprotein. Materials and Methods: A 26-year-old male patient infected with HCV 10 years ago was followed up. After 9 years of chronic infection. The patient had managed to resolve the infection for a period of 9 months, after which the patient experienced a viral recurrence characterized by high viral load and diverse HCV quasispecies. The IRES structures of the viral strains that disappeared were comparable with those that are currently active using structural mutational analysis. Results: A novo mutational position 254 combined with a rarely observed mutation at position 253 in the stem of the IIId subdomain were observed and the new conformation had an octa-apical loop (AGUGUUGG) and a shift in the 3 ` GU from the loop to the stem. Conclusions: These mutations were found to be highly deleterious, and they affected the direct binding of the IIId loop to the 40S ribosomal subunit with a subsequent inhibition of translation of viral polyprotein and clearance of the virus.

How to cite this article:
Bader El Din N G, El Hefnawy M M, Omran M H, Dawood R M, El Abd Y, Ibrahim M K, El Awady M K. Spontaneous clearance of chronic hepatitis C infection is associated with an internal ribosomal entry site IIId stem loop structure variant .Indian J Med Microbiol 2015;33:143-148

How to cite this URL:
Bader El Din N G, El Hefnawy M M, Omran M H, Dawood R M, El Abd Y, Ibrahim M K, El Awady M K. Spontaneous clearance of chronic hepatitis C infection is associated with an internal ribosomal entry site IIId stem loop structure variant . Indian J Med Microbiol [serial online] 2015 [cited 2020 Aug 8 ];33:143-148
Available from: http://www.ijmm.org/article.asp?issn=0255-0857;year=2015;volume=33;issue=5;spage=143;epage=148;aulast=Bader;type=0