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Year : 2015  |  Volume : 33  |  Issue : 4  |  Page : 568--571

Loop mediated isothermal amplification assay using hydroxy naphthol blue, conventional polymerase chain reaction and real-time PCR in the diagnosis of intraocular tuberculosis

PK Balne1, S Basu2, S Rath2, MR Barik1, S Sharma3 
1 Ocular Microbiology Service, L V Prasad Eye Institute, Bhubaneswar, Odisha, India
2 Retina-Vitreous and Uveitis Services, L V Prasad Eye Institute, Bhubaneswar, Odisha, India
3 Jhaveri Microbiology Centre, L V Prasad Eye Institute, Hyderabad, Telangana, India

Correspondence Address:
S Sharma
Jhaveri Microbiology Centre, L V Prasad Eye Institute, Hyderabad, Telangana
India

This study is a comparative evaluation (Chi-square test) of a closed tube loop mediated isothermal amplification assay using hydroxy naphthol blue dye (HNB-LAMP), real-time polymerase chain reaction (PCR) and conventional PCR in the diagnosis of intraocular tuberculosis. Considering clinical presentation as the gold standard in 33 patients, the sensitivity of HNB-LAMP assay (75.8%) was higher (not significant, P value 0.2) than conventional PCR (57.6%) and lower than real-time PCR (90.9%). Specificity was 100% by all three methods. No amplification was observed in negative controls (n = 20) by all three methods. The cost of the HNB-LAMP assay was Rs. 500.00 and it does not require thermocycler, therefore, it can be used as an alternative to conventional PCR in resource-poor settings.

How to cite this article:
Balne P K, Basu S, Rath S, Barik M R, Sharma S. Loop mediated isothermal amplification assay using hydroxy naphthol blue, conventional polymerase chain reaction and real-time PCR in the diagnosis of intraocular tuberculosis.Indian J Med Microbiol 2015;33:568-571

How to cite this URL:
Balne P K, Basu S, Rath S, Barik M R, Sharma S. Loop mediated isothermal amplification assay using hydroxy naphthol blue, conventional polymerase chain reaction and real-time PCR in the diagnosis of intraocular tuberculosis. Indian J Med Microbiol [serial online] 2015 [cited 2020 Aug 5 ];33:568-571
Available from: http://www.ijmm.org/article.asp?issn=0255-0857;year=2015;volume=33;issue=4;spage=568;epage=571;aulast=Balne;type=0