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Year : 2015  |  Volume : 33  |  Issue : 4  |  Page : 482--490

How to develop an in-house real-time quantitative cytomegalovirus polymerase chain reaction: Insights from a cancer centre in Eastern India

Anusha Harishankar, Mammen Chandy, Sanjay Bhattacharya 
 Departments of Microbiology and Clinical Hematology, Tata Medical Center, Rajarhat, Kolkata, India

Correspondence Address:
Sanjay Bhattacharya
Departments of Microbiology and Clinical Hematology, Tata Medical Center, Rajarhat, Kolkata
India

Development of a reliable, cost-effective cytomegalovirus quantitative polymerase chain reaction (QPCR) is a priority for developing countries. Manufactured kits are expensive, and availability can be inconsistent. Development of an in-house QPCR kit that is reliable and quality assured requires significant effort and initial investment. However, the rewards of such an enterprise are manifold and include an in-depth understanding of molecular reactions, and expertise in the development of further low-cost molecular kits. The experience of an oncology centre in Eastern India has been shared. Hopefully, this would provide a brief roadmap for such an initiative. Staff with adequate understanding of molecular processes are essential along with vital infrastructure for molecular research and development.

How to cite this article:
Harishankar A, Chandy M, Bhattacharya S. How to develop an in-house real-time quantitative cytomegalovirus polymerase chain reaction: Insights from a cancer centre in Eastern India.Indian J Med Microbiol 2015;33:482-490

How to cite this URL:
Harishankar A, Chandy M, Bhattacharya S. How to develop an in-house real-time quantitative cytomegalovirus polymerase chain reaction: Insights from a cancer centre in Eastern India. Indian J Med Microbiol [serial online] 2015 [cited 2020 Sep 26 ];33:482-490
Available from: http://www.ijmm.org/article.asp?issn=0255-0857;year=2015;volume=33;issue=4;spage=482;epage=490;aulast=Harishankar;type=0