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Year : 2014  |  Volume : 32  |  Issue : 2  |  Page : 148-

PCR analysis of 16S-23S ribosomal RNA internal transcribed spacer for identification of Acinetobacter clinical isolates

V Wiwanitkit 
 Hainan Medical University, China, Visiting Professor, Faculty of Medicine, University of Nis, Serbia, Adjunct Professor, Joseph Ayobabalola University, Nigeria

Correspondence Address:
V Wiwanitkit
Hainan Medical University, China, Visiting Professor, Faculty of Medicine, University of Nis, Serbia, Adjunct Professor, Joseph Ayobabalola University
Nigeria

How to cite this article:
Wiwanitkit V. PCR analysis of 16S-23S ribosomal RNA internal transcribed spacer for identification of Acinetobacter clinical isolates.Indian J Med Microbiol 2014;32:148-148

How to cite this URL:
Wiwanitkit V. PCR analysis of 16S-23S ribosomal RNA internal transcribed spacer for identification of Acinetobacter clinical isolates. Indian J Med Microbiol [serial online] 2014 [cited 2020 Jul 4 ];32:148-148
Available from: http://www.ijmm.org/text.asp?2014/32/2/148/129798

Full Text

The genus Acinetobacter comprises many clinical important species. [1] The identification of the problematic species is very important in clinical practice. Within the few years, there is an increased consideration on the clinically important Acinetobacter. According to a recent report from a hospital in India, multi-drug resistant Acinetobacter species (9%) became an uprising important nosocomial pathogen and the rapid diagnosis and prompt treatment is the key for successful clinical management. [2] The advanced molecular biology technique can help discriminate between problematic and non-problematic species, which is better than the classical microbiological technique that has limitation in discrimination. Focusing on the classical technique, the problem of 'unidentification' can be seen and this becomes the reason for the requirement of the new diagnostic technique. [3]

As already mentioned, the applied molecular biology technique can help identify the Acinetobacter species. An important method is the use of polymerase chain reaction (PCR) to detect. The focus on the high conservation of primary and secondary structures within species, ribosomal RNA genes (16S and 23S) is mainly used. [4] According to the report by Garcνa-Arata et al., it is confirmed that 'PCR-amplified 16S and 23S rDNA restriction analysis is both an accurate and rapid method for the identification of Acinetobacter at the DNA group level'. [5] In a more detail, of several PCR-based techniques, the best method is repetitive extragenic palindromic PCR. [6]

Due to the increasing problem of problematic strains of Acinetobacter, the setting of the newly diagnostic tool is very important. The present report in the Indian Journal of Medical Microbiology can be a good example showing advantage of the newly developed PCR tool for identifying newly emerged strain of Acinetobacter clinical isolates. [7] The present report follows the basic theory for setting of a PCR system based on 16S and 23S rDNA internal transcribed spacer (ITS), which is proved to provide accuracy result. In fact, this report shows and implies that the newly developed PCR tool should be available in medical laboratory. This should be considered in any clinical microbiology laboratory. There are some points to be considered in setting of such laboratory including (a) the setting has to follow the standard clinical microbiology practice guideline, (b) standard evaluation of the newly set tool based on diagnostic test evaluation is needed and (c) the cost effectiveness analysis of the newly set tool should also be done. Also, in actual usage, the good quality control covering all phases (pre-, intra- and post-analytical phases) is needed. Finally, the future trend of diagnosis of Acinetobacter species should be mentioned. A more advanced technique, microarray, is the hope for fast detection of the antibiotic resistance. [8]

References

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5García-Arata MI, Gerner-Smidt P, Baquero F, Ibrahim A. PCR-amplified 16S and 23S rDNA restriction analysis for the identification of Acinetobacter strains at the DNA group level. Res Microbiol 1997;148:777-84.
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7Identification of Acinetobacter clinical isolates by polymerase chain reaction analysis of 16S-23S ribosomal ribonucleic acid internal transcribed spacer. Indian J Med Microbiol 2014.32:149-53.
8Coyne S, Guigon G, Courvalin P, Périchon B. Screening and quantification of the expression of antibiotic resistance genes in Acinetobacter baumannii with a microarray. Antimicrob Agents Chemother 2010;54:333-40.