Indian Journal of Medical Microbiology Home 

ORIGINAL ARTICLE
[View FULLTEXT] [Download PDF]
Year : 2012  |  Volume : 30  |  Issue : 2  |  Page : 159--164

The non-association of Panton-Valentine leukocidin and mecA genes in the genome of Staphylococcus aureus from hospitals in South Western Nigeria

OA Terry Alli, DO Ogbolu, JO Mustapha, R Akinbami, AO Ajayi 
 Department of Biomedical Sciences, College of Health Sciences, LadokeAkintola University of Technology, Osogbo Campus, Nigeria

Correspondence Address:
O A Terry Alli
Department of Biomedical Sciences, College of Health Sciences, LadokeAkintola University of Technology, Osogbo Campus
Nigeria

Purpose: Virulence genes play important roles in pathogenesis of infections caused by S. aureus. The aim of this study was to determine the prevalence of PVL, eta and mecA genes in S. aureus isolated from patients in South-Western Nigeria. Materials and Methods: In this study, a total of 116 S. aureus isolates from the clinical specimens submitted to laboratories in tertiary hospitals in the South Western Nigeria were used. Antibiotic susceptibility test was carried out to determine the susceptibility pattern of the isolates using multiple antibiotics disc. Minimum inhibitory concentration (MIC) was also carried out to determine the degree of resistant of the isolates to methicillin. PCR was used to screen for the presence of PVL, eta, and mecAgenes. Results:mecA gene was detected in 48 (41.4%) of 116 strains of S. aureus. The MIC 50 and MIC 90 for mecA negative strains were 1 and 8 μg/ml, respectively while the MIC 50 and MIC 90 for mecA positive were >256 μg/ml. Twenty eight (24.1%) of 116 isolates were PVL gene positive with none of them mecA+. The prevalence of community acquired MRSA (CA-MRSA) was estimated to be 6.9% using molecular techniques. No localization of mecA gene and PVL gene on the genome of the entire S. aureus strains studied. Site of isolation of organism /specimen type was found to be associated with the prevalence of PVL+ and mecA+ S. aureus (P< 0.01). Conclusion: This study concludes that the PVL+ MRSA is rare and the prevalence of CA-MRSA is low in South-Western, Nigeria.

How to cite this article:
Terry Alli O A, Ogbolu D O, Mustapha J O, Akinbami R, Ajayi A O. The non-association of Panton-Valentine leukocidin and mecA genes in the genome of Staphylococcus aureus from hospitals in South Western Nigeria.Indian J Med Microbiol 2012;30:159-164

How to cite this URL:
Terry Alli O A, Ogbolu D O, Mustapha J O, Akinbami R, Ajayi A O. The non-association of Panton-Valentine leukocidin and mecA genes in the genome of Staphylococcus aureus from hospitals in South Western Nigeria. Indian J Med Microbiol [serial online] 2012 [cited 2019 Jul 17 ];30:159-164
Available from: http://www.ijmm.org/article.asp?issn=0255-0857;year=2012;volume=30;issue=2;spage=159;epage=164;aulast=Terry;type=0