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Year : 2012  |  Volume : 30  |  Issue : 1  |  Page : 64--68

Evaluation of the microscopic observational drug susceptibility assay for rapid and efficient diagnosis of multi-drug resistant tuberculosis

RP Lazarus1, S Kalaiselvan1, KR John2, JS Michael1 
1 Department of Microbiology, Christian Medical College, Vellore, 632004, Tamil Nadu, India
2 Department of Community Medicine, Christian Medical College, Vellore, 632004, Tamil Nadu, India

Correspondence Address:
J S Michael
Department of Microbiology, Christian Medical College, Vellore, 632004, Tamil Nadu
India

Purpose: Tuberculosis (TB) is endemic in India and the burden of multi-drug-resistant tuberculosis (MDR-TB) is high. Early detection of MDR-TB is of primary importance in controlling the spread of TB. The microscopic observational drug susceptibility (MODS) assay has been described as a cost-effective and rapid method by which mycobacterial culture and the drug susceptibility test (DST) can be done at the same time. Materials and Methods: A total of 302 consecutive sputum samples that were received in an accredited mycobacteriology laboratory for conventional culture and DST were evaluated by the MODS assay. Results: In comparison with conventional culture on Lowenstein Jensen (LJ) media, the MODS assay showed a sensitivity of 94.12% and a specificity of 89.39% and its concordance with the DST by the proportion method on LJ media to isoniazid and rifampicin was 90.8% and 91.5%, respectively. The turnaround time for results by MODS was 9 days compared to 21 days by culture on LJ media and an additional 42 days for DST by the 1% proportion method. The cost of performing a single MODS assay was Rs. 250/-, compared to Rs. 950/- for culture and 1st line DST on LJ. Conclusion: MODS was found to be a sensitive and rapid alternative method for performing culture and DST to identify MDR-TB in resource poor settings.

How to cite this article:
Lazarus R P, Kalaiselvan S, John K R, Michael J S. Evaluation of the microscopic observational drug susceptibility assay for rapid and efficient diagnosis of multi-drug resistant tuberculosis.Indian J Med Microbiol 2012;30:64-68

How to cite this URL:
Lazarus R P, Kalaiselvan S, John K R, Michael J S. Evaluation of the microscopic observational drug susceptibility assay for rapid and efficient diagnosis of multi-drug resistant tuberculosis. Indian J Med Microbiol [serial online] 2012 [cited 2020 Aug 14 ];30:64-68
Available from: http://www.ijmm.org/article.asp?issn=0255-0857;year=2012;volume=30;issue=1;spage=64;epage=68;aulast=Lazarus;type=0