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Year : 2010  |  Volume : 28  |  Issue : 3  |  Page : 241--244

Detection and characterization of metallo beta lactamases producing Pseudomonas aeruginosa

A Manoharan1, S Chatterjee1, D Mathai1, SARI Study Group2 
1 Benjamin M Pulimood Laboratories for Infection, Immunity and Inflammation (BMPLIII), Department of Medicine Unit I and Infectious Diseases, Christian Medical College, Vellore -632004, Tamil Nadu, India
2 Surveillance of Antimicrobial Resistance in India Study Group (SARISG), Kothari Medical Center, Kolkata - Dr. Anuradha Aggarwal; Mahatma Gandhi Institute of Medical Sciences, Wardha - Dr. Vijayshree Deotale & Dr. D. K. Mendiratta; P D Hinduja Hospital, Mumbai - Dr. Camilla Rodriguez; Sri Ramachandra Medical College, Chennai - Dr. Padma Srikanth; Sir Gangaram Hospital, New Delhi - Dr. Chand Wattal; Sundaram Medical Center, Chennai - Dr. Vaidehi & Dr. Ram Rajagopal; Tata Memorial Center, Mumbai - Dr. Rohini Kelkar, India

Correspondence Address:
A Manoharan
Benjamin M Pulimood Laboratories for Infection, Immunity and Inflammation (BMPLIII), Department of Medicine Unit I and Infectious Diseases, Christian Medical College, Vellore -632004, Tamil Nadu
India

This study was undertaken to evaluate phenotypic and genotypic methods for detection of Metallo-Beta-Lactamases (MBLs) among nosocomial Pseudomonas aeruginosa. Sixty one among 176 P. aeruginosa isolates, collected as part of a multicentric study (2005-2007), were evaluated for carbapenem resistance (CARB-R; resistant to either imipenem/meropenem) and screened for MBL by Combination Disk Diffusion Test (CDDT) using imipenem (IMP), meropenem (MER) and ceftazidime (CAZ) with EDTA. MBL positives were further confirmed by IMP + EDTA Etest. Twenty strains (42.6%) were found to be MBL producers among the 61 P. aeruginosa. PCR for IMP and VIM MBL was performed on 48 of the 61, 15 were positive for VIM MBL type. CDDT using IMP + EDTA had the highest sensitivity and specificity of 87.8% and 84.4% when compared to Etest, which was higher than the values obtained for CAZ + EDTA and MER + EDTA. CDDT using IMP + EDTA also compared very well with the PCR (specificity = 90.9%, sensitivity = 93.3%). CARB-R among P. aeruginosa is mediated predominantly via MBL production. Clinical P. aeruginosa isolates can be screened routinely using the less expensive IMP + EDTA CDDT in clinical microbiology laboratories.

How to cite this article:
Manoharan A, Chatterjee S, Mathai D, SARI Study Group. Detection and characterization of metallo beta lactamases producing Pseudomonas aeruginosa.Indian J Med Microbiol 2010;28:241-244

How to cite this URL:
Manoharan A, Chatterjee S, Mathai D, SARI Study Group. Detection and characterization of metallo beta lactamases producing Pseudomonas aeruginosa. Indian J Med Microbiol [serial online] 2010 [cited 2020 Jan 20 ];28:241-244
Available from: http://www.ijmm.org/article.asp?issn=0255-0857;year=2010;volume=28;issue=3;spage=241;epage=244;aulast=Manoharan;type=0