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Year : 2010  |  Volume : 28  |  Issue : 3  |  Page : 238--240

Comparison of an in-house latex agglutination test with IgM ELISA and MAT in the diagnosis of leptospirosis

S Shekatkar, NS Acharya, BN Harish, SC Parija 
 Department of Microbiology, JIPMER, Pondicherry, India

Correspondence Address:
S Shekatkar
Department of Microbiology, JIPMER, Pondicherry
India

The laboratory diagnosis of leptospirosis is fraught with several problems. Isolation of Leptospira by culture has a low sensitivity and the microscopic agglutination test (MAT) is time consuming To overcome these problems, a rapid latex agglutination test (LAT) has been standardized for the detection of antileptospiral antibodies in serum samples from suspected cases of leptospirosis. We compared the efficiency of the LAT to a commercially available IgM ELISA and MAT. A total of 150 serum samples were tested by LAT, IgM ELISA, and MAT. The positivity was 26.7%, 26% and 24% respectively. The sensitivity and specificity of LAT as compared to MAT was 90.62 and 91.96% respectively. Even though LAT and ELISA showed similar results, its rapidity and simplicity made latex agglutination test more suitable as a rapid screening test.

How to cite this article:
Shekatkar S, Acharya N S, Harish B N, Parija S C. Comparison of an in-house latex agglutination test with IgM ELISA and MAT in the diagnosis of leptospirosis.Indian J Med Microbiol 2010;28:238-240

How to cite this URL:
Shekatkar S, Acharya N S, Harish B N, Parija S C. Comparison of an in-house latex agglutination test with IgM ELISA and MAT in the diagnosis of leptospirosis. Indian J Med Microbiol [serial online] 2010 [cited 2019 Oct 19 ];28:238-240
Available from: http://www.ijmm.org/article.asp?issn=0255-0857;year=2010;volume=28;issue=3;spage=238;epage=240;aulast=Shekatkar;type=0