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Year : 2010  |  Volume : 28  |  Issue : 2  |  Page : 147--151

Identification of Candida species using PCR-RFLP in cancer patients in Iran

T Shokohi1, MB Hashemi Soteh2, Z Saltanat Pouri1, MT Hedayati1, S Mayahi1 
1 Department of Medical Mycology and Parasitology , Sari Medical School, Mazandaran University of Medical Sciences, Sari, Iran
2 Department of Biochemistry and Genetics, Sari Medical School, Mazandaran University of Medical Sciences, Sari, Iran

Correspondence Address:
T Shokohi
Department of Medical Mycology and Parasitology , Sari Medical School, Mazandaran University of Medical Sciences, Sari
Iran

Opportunistic infections caused by Non-Candida albicans. have been increasing. Traditional methods that are used to identify clinical isolates of Candida species are time-consuming and not appropriate for rapid, accurate and reliable identification. Purpose: To identify Candida spp isolated from cancer patients using PCR-restriction enzyme. Materials and ethods: Using universal primers, ITS1 and ITS4, in this study, we could amplify ITS1-5.8S-ITS2 rDNA regions at both 80 clinical isolates and 3 standard strains. The PCR products were digested with two restriction enzymes MspI and BlnI separately. Result: We successfully identified all isolated species using two restriction enzymes (MspI, BlnI). Candida albicans was the most common species (77.5%), followed by C. glabrata (15%), C. tropicalis (5%), C. krusei (2.5%). Although the primers and enzyme had the ability to identify C. parapsilosis, C. guilliermondii, C. dubliniensis, present isolates did not include these among identified ones. Conclusion: RFLP-PCR using ITSI and ITS4 primers and restriction enzyme is a rapid, easy, reliable and also applicable method in clinical laboratory for identification of medically important Candida spp.

How to cite this article:
Shokohi T, Hashemi Soteh M B, Pouri Z S, Hedayati M T, Mayahi S. Identification of Candida species using PCR-RFLP in cancer patients in Iran.Indian J Med Microbiol 2010;28:147-151

How to cite this URL:
Shokohi T, Hashemi Soteh M B, Pouri Z S, Hedayati M T, Mayahi S. Identification of Candida species using PCR-RFLP in cancer patients in Iran. Indian J Med Microbiol [serial online] 2010 [cited 2020 Aug 8 ];28:147-151
Available from: http://www.ijmm.org/article.asp?issn=0255-0857;year=2010;volume=28;issue=2;spage=147;epage=151;aulast=Shokohi;type=0