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Year : 2009  |  Volume : 27  |  Issue : 4  |  Page : 325--328

Effect of stress on production of heat labile enterotoxin by Escherichia coli

A Hegde, GK Bhat, S Mallya 
 Department of Microbiology, Kasturba Medical College, Mangalore - 575 001, India

Correspondence Address:
A Hegde
Department of Microbiology, Kasturba Medical College, Mangalore - 575 001
India

Enterotoxigenic Escherichia coli (ETEC) is an important pathogen responsible for secretory diarrhoea. The production of heat labile enterotoxin (LT), by ETEC, is largely responsible for the pathogenesis of diarrhoea. In the present study we investigated the effect of stress factors such as temperature, pH, osmotic stress and nutritional limitation on the production of LT by ETEC using in-house GMI-ELISA. Four strains of E. coli consisting, one standard strain MTCC 723 and three clinical isolates were used in the study. Maximum amount of LT (OD 3.285) was produced at 37 0 C followed by 40 0 C (OD 3.305). Growth of E. coli in medium with pH 8.6 resulted in maximum amount of LT production (OD 3.489). LT was not detectable when bacteria were grown in medium with pH ≤7.2 and ≥ 9.2. Sodium chloride concentration of 0.2 M stimulated maximum amount of LT production. Maximum amount of LT was produced when the bacteria were grown in medium containing 2.5g/l of glucose. All the stress factors had a significant effect on the LT production by E. coli , though quantitative differences in the various strains were observed.

How to cite this article:
Hegde A, Bhat G K, Mallya S. Effect of stress on production of heat labile enterotoxin by Escherichia coli.Indian J Med Microbiol 2009;27:325-328

How to cite this URL:
Hegde A, Bhat G K, Mallya S. Effect of stress on production of heat labile enterotoxin by Escherichia coli. Indian J Med Microbiol [serial online] 2009 [cited 2019 Oct 14 ];27:325-328
Available from: http://www.ijmm.org/article.asp?issn=0255-0857;year=2009;volume=27;issue=4;spage=325;epage=328;aulast=Hegde;type=0