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Year : 2009  |  Volume : 27  |  Issue : 4  |  Page : 311--320

Genetic diversity among toxigenic clostridia isolated from soil, water, meat and associated polluted sites in South India

S Sathish, K Swaminathan 
 Department of Microbial Biotechnology, Bharathiar University, Coimbatore - 641 046, Tamilnadu, India

Correspondence Address:
S Sathish
Department of Microbial Biotechnology, Bharathiar University, Coimbatore - 641 046, Tamilnadu
India

Purpose: To investigate the genetic diversity of toxigenic Clostridium strains isolated from soil, water, meat and its associated polluted sites of Southern India. Materials and Methods: A total of 27 identified isolates of six different toxigenic clostridial species including C. bifermentans , C. botulinum , C. chauvoei , C. ramosum , C. tetani and C. novyi were isolated and characterized by conventional DNA restriction digestion analysis (REA) and by whole-cell and excretory protein patterns on SDS-PAGE. Results: The DNA fragment size ranged from 35-160 kilobases and the protein bands 30-200 KDa, followed by numerical analyses and phylogenetic analyses. Whole-cell protein banding pattern were unique with strains of C. chauvoei , C. novyi and C. ramosum . All the strains were heterogeneous and distinct in restriction digestion pattern and excretory protein patterns. Conclusion: These analyses contribute to the understanding of prevalence of toxigenic clostridial species and phylogeny within the species and assist in development of improved diagnostics and therapeutics for the treatment of clostridial infections.

How to cite this article:
Sathish S, Swaminathan K. Genetic diversity among toxigenic clostridia isolated from soil, water, meat and associated polluted sites in South India.Indian J Med Microbiol 2009;27:311-320

How to cite this URL:
Sathish S, Swaminathan K. Genetic diversity among toxigenic clostridia isolated from soil, water, meat and associated polluted sites in South India. Indian J Med Microbiol [serial online] 2009 [cited 2020 Sep 20 ];27:311-320
Available from: http://www.ijmm.org/article.asp?issn=0255-0857;year=2009;volume=27;issue=4;spage=311;epage=320;aulast=Sathish;type=0