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Year : 2009  |  Volume : 27  |  Issue : 2  |  Page : 107--110

Role of azithromycin against clinical isolates of family enterobacteriaceae: A comparison of its minimum inhibitory concentration by three different methods

N Chayani, S Tiwari, G Sarangi, B Mallick, A Mohapatra, BP Paty, P Das 
 Department of Microbiology, S.C.B. Medical College, Cuttack -753 007, India

Correspondence Address:
N Chayani
Department of Microbiology, S.C.B. Medical College, Cuttack -753 007
India

Purpose: To determine the effect of azithromycin, a new azalide antibiotic, on clinical isolates of the family Enterobacteriaceae and to determine and compare its minimum inhibitory concentration (MIC) by disk diffusion, agar dilution and E-test methods. Materials and Methods: One hundred fifty-nine bacterial strains belonging to the family Enterobacteriaceae, isolated from different clinical samples, were tested for their susceptibility to azithromycin by disk diffusion, agar dilution and E-test methods. The MIC values were analysed and the percentages of agreement between the different methods were mentioned. Results: Of the 159 isolates of the family Enterobacteriaceae, 60.37% were E. coli followed by Klebsiella species 28.3%, Salmonella and Shigella species 3.77% and Enterobacter and Citrobacter species 1.88% each. Maximum isolates were obtained from urine 117/159 (73.58%). Azithromycin was found to be more active against Salmonella and Shigella species, showing 100% sensitivity the by E-test and 83.33% by the disk diffusion methods. In the agar dilution method, 83.33% of Salmonella and 66.66% of Shigella species were sensitive to azithromycin. The overall agreement between disk diffusion and agar dilution method was 96.8%, between agar dilution and E-test was 88% and between disk diffusion and E-test was 91.2%. Conclusion: Azithromycin may become an important addition to our antimicrobial strategies, especially for the treatment of bacterial diarrhoea and infections caused by Salmonella typhi.

How to cite this article:
Chayani N, Tiwari S, Sarangi G, Mallick B, Mohapatra A, Paty B P, Das P. Role of azithromycin against clinical isolates of family enterobacteriaceae: A comparison of its minimum inhibitory concentration by three different methods.Indian J Med Microbiol 2009;27:107-110

How to cite this URL:
Chayani N, Tiwari S, Sarangi G, Mallick B, Mohapatra A, Paty B P, Das P. Role of azithromycin against clinical isolates of family enterobacteriaceae: A comparison of its minimum inhibitory concentration by three different methods. Indian J Med Microbiol [serial online] 2009 [cited 2020 Feb 21 ];27:107-110
Available from: http://www.ijmm.org/article.asp?issn=0255-0857;year=2009;volume=27;issue=2;spage=107;epage=110;aulast=Chayani;type=0