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Year : 2009  |  Volume : 27  |  Issue : 1  |  Page : 12--16

Prevalence of hepatitis C virus (HCV) coinfection in HIV infected individuals in south India and characterization of HCV genotypes

SPD Ponamgi1, S Rahamathulla1, YN Kumar1, M Chandra1, N Lakshmi2, CM Habibullah1, MN Khaja1 
1 Center for Liver Research and Diagnostics, Deccan College of Medical Sciences and Allied Hospitals, Kanchanbagh, Hyderabad-500 058, Andhra Pradesh, India
2 Department of Biotechnology, Jawaharlal Nehru Technology University, Hyderabad-500 072, Andhra Pradesh, India

Correspondence Address:
M N Khaja
Center for Liver Research and Diagnostics, Deccan College of Medical Sciences and Allied Hospitals, Kanchanbagh, Hyderabad-500 058, Andhra Pradesh
India

Purpose: To determine anti-HCV antibodies and genomic subtype of HCV in 1487 confirmed human immunodeficiency virus (HIV) positive samples. Methods: A total of 1487 confirmed HIV-positive samples were tested for anti-HCV antibodies by using a third generation ELISA kit (Ortho 3.0) and by RT PCR for HCV. HIV and HCV coinfected samples were selected for HCV genotyping by RFLP and subtyping with NS5-type specific primers. Results: A total of 1487 HIV-infected serum samples were screened for HCV infection, of which, a 1443 (97.04%) were negative and 45 (3.02%) were coinfected. HIV-HCV coinfection was predominant in the age group 41-50 years (51.1%). HCV genotyping and subtyping was done for the 45 HCV RNA-positive specimens of which genotype 1 was observed in 31 (68.8%) and genotype 3 was observed in 14 (31.1%) subjects. Further subtyping analysis showed the genotype 1b in 23 (51.1%), 1a in eight (17.7%), 3a in 10 (22.2%) and 3b in four (8.8%) subjects. Conclusion: HIV and HCV seroprevalence is higher in South India, and the most prevalent genotype in coinfection was genotype 1b.

How to cite this article:
Ponamgi S, Rahamathulla S, Kumar Y N, Chandra M, Lakshmi N, Habibullah C M, Khaja M N. Prevalence of hepatitis C virus (HCV) coinfection in HIV infected individuals in south India and characterization of HCV genotypes.Indian J Med Microbiol 2009;27:12-16

How to cite this URL:
Ponamgi S, Rahamathulla S, Kumar Y N, Chandra M, Lakshmi N, Habibullah C M, Khaja M N. Prevalence of hepatitis C virus (HCV) coinfection in HIV infected individuals in south India and characterization of HCV genotypes. Indian J Med Microbiol [serial online] 2009 [cited 2020 Aug 10 ];27:12-16
Available from: http://www.ijmm.org/article.asp?issn=0255-0857;year=2009;volume=27;issue=1;spage=12;epage=16;aulast=Ponamgi;type=0