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|Year : 2008 | Volume
| Issue : 2 | Page : 187--189
Gastrointestinal histoplasmosis presenting as colonic pseudotumour
S Sehgal, R Chawla, PS Loomba, B Mishra
Department of Microbiology, GB Pant Hospital, New Delhi - 110 002, India
Department of Microbiology, GB Pant Hospital, New Delhi - 110 002
We report a case of gastrointestinal histoplasmosis in a 45-year-old HIV positive man who was misdiagnosed as a case of colonic cancer. The patient presented with low-grade fever, pain in lower abdomen, anorexia and weight loss of six months duration. On examination a lump in the left iliac fossa was detected. Colonoscopy revealed stricture and ulcerated growth in the sigmoid colon. Radiological investigations suggested malignant/inflammatory mass in the sigmoid colon with luminal compromise. Patient was operated and ulcerated tissue was sent for histopathological examination, which revealed numerous intracellular, 2-4 μm, oval, narrow-based budding yeast cells suggestive of Histoplasma capsulatum . Subsequently, the patient developed fluffy opacities on X-ray chest. Examination of sputum revealed presence of acid-fast bacilli and yeast forms of H. capsulatum . Patient was started on amphotericin B but died on the seventeenth postoperative day. The diagnosis of histoplasmosis was made retrospectively. Atypical presentation and rarity of the disease led to this diagnostic pitfall. To the best of our knowledge this is the first report of gastrointestinal histoplasmosis presenting as colonic pseudotumour from India.
|How to cite this article:|
Sehgal S, Chawla R, Loomba P S, Mishra B. Gastrointestinal histoplasmosis presenting as colonic pseudotumour.Indian J Med Microbiol 2008;26:187-189
|How to cite this URL:|
Sehgal S, Chawla R, Loomba P S, Mishra B. Gastrointestinal histoplasmosis presenting as colonic pseudotumour. Indian J Med Microbiol [serial online] 2008 [cited 2020 Sep 26 ];26:187-189
Available from: http://www.ijmm.org/text.asp?2008/26/2/187/40541
Histoplasmosis is a granulomatous disease caused by the intracellular dimorphic fungus Histoplasma capsulatum .  H. capsulatum is found most commonly in North America and Central America, but the fungus has been reported from diverse areas around the world. In endemic areas infection occurs during day-to-day activities that lead to disruption and subsequent inhalation of soil contaminated with bird or bat guano. In areas endemic to histoplasmosis the vast majority of infections are either asymptomatic or present as a mild illness that is never recognized as being histoplasmosis.  Clinically, histoplasmosis may present as a pulmonary (acute pulmonary histoplasmosis, chronic cavitary pulmonary histoplasmosis, granulomatous mediastinitis and mediastinal fibrosis) or a disseminated infection.  Disseminated histoplasmosis has become increasingly important with the advent of new and expanding population of immunocompromised patients. Although respiratory tract is the primary site of the disease, virtually all organs rich in mononuclear cells can be infected.  Primary gastrointestinal infections are an uncommon presentation of the infection, almost always associated with disseminated form of the disease and/or immunodeficiency.  Symptomatic gastrointestinal tract infection is more common in AIDS patients and can mimic other AIDS associated opportunistic infections that cause diarrhoea. The colon is the most commonly involved site followed by small bowel with lesions presenting as ulcerations, polyps, strictures or perforations. 
A 45-year-old male patient working as a supervisor in a factory presented in the Gastrointestinal surgery OPD in March 2007 with complaints of low-grade fever, pain in lower abdomen, anorexia and weight loss for the last six months. There was no history of constipation, jaundice, haematemesis or melaena. The patient was non-diabetic and normotensive with no past history of tuberculosis.
On general physical examination, pallor was present; however, there was no icterus, cyanosis, clubbing, pedal oedema or lymphadenopathy. On per abdominal examination, mildly tender lump was present in left iliac fossa. Rest of the abdomen was soft and there was no organomegaly. The rest of the systemic examination was normal. The patient was advised computed tomography (CT) of abdomen, colonoscopy with biopsy, barium enema and ultrasonography (USG) of abdomen. CT of the abdomen conducted in March 2007 revealed thick sigmoid colon and descending colon with luminal compromise. Colonoscopy with biopsy was carried out in April 2007 that revealed stricture and ulceronodular growth in sigmoid colon at 10-15 cm from anus. Biopsy taken during colonoscopy showed acute severe colitis and Ziehl-Neelsen staining of the tissue was negative for acid-fast bacilli. Barium enema conducted in April 2007 showed asymmetric thickening of the sigmoid colon with apple core appearance with shouldering of both ends suggestive of malignant or inflammatory mass. No abnormality was detected on USG of abdomen done in May 2007. Based on the above findings a diagnosis of carcinoma of sigmoid colon was made and elective surgery was planned. Chest X-ray and other investigations carried out on 14 th May 2007 as part of the pre-anaesthesia check-up were normal. The patient was admitted in the gastrointestinal surgery ward on 16 th May 2007 and was operated on 24 th May 2007. During the operation segmental sigmoid colectomy with end to side colo-colic anastomosis was performed. The immediate postoperative period was uneventful.
Histopathological examination of the resected segment of the sigmoid colon revealed numerous intracellular, 2-4 μm, oval, narrow-based budding yeast cells suggestive of H. capsulatum [Figure 1]. Fluffy opacities in both the lung fields were seen on a repeat chest X-ray done on 30 th May 2007. In view of the above findings serum specimen (for HIV serology), sputum specimen (for microscopy and for fungal and mycobacterial culture) and blood specimen (for fungal culture) were collected. Anti-HIV antibody was positive by ELISA (ERBA LISA HIV1 + 2, Erba Diagnostics, Mannheim, Germany), immunochromatographic test (Anti-HIV Triline Cassette Serum/ Whole Blood Test, Ind Diagnostic Inc, Delta B.C, Canada) and dot immunoassay (CombAids-RS, Span Diagnostics Ltd, Surat, India). Giemsa staining of sputum showed intracellular, 2-4 μm, oval, narrow-based budding yeast cells suggestive of H. capsulatum [Figure 2]. Ziehl-Neelsen staining of sputum showed presence of acid-fast bacilli. Culture of sputum grew Mycobacterium tuberculosis . Fungal cultures of sputum and blood specimens did not yield H. capsulatum . Patient was treated with intravenous lyophilized amphotericin B, however the patient's condition deteriorated steadily and he died on the 17 th post-operative day.
Histoplasmosis has been reported both in immunocompetent as well as immunocompromised patients with disseminated forms being more common in the latter group.  In India, infection with H. capsulatum is infrequent, being reported sporadically from different parts of the country. , Unlike in the US no clearly demarcated endemic foci have been identified in India. However, a large number of cases in India have been reported from Eastern India. 
In HIV positive patients the prevalence of histoplasmosis varies from 5% to 32% depending on the endemicity of the disease.  In HIV infected patients, working in chicken coops, CD4 count 
In India, Subramanian et al. retrospectively analysed 19 cases of disseminated histoplasmosis diagnosed during a 10-year period. The authors found diabetes mellitus and HIV infection to be the most common co-morbid conditions. Weight loss and fever were found to be the most common symptoms while on physical examination; oropharyngeal ulcers, hepatosplenomegaly and lymphadenopathy were the most common signs. Out of three patients who had gastrointestinal tract infection, two had ulcerative lesions in the rectum, ileum and oesophagus while one had multiple intestinal hemorrhages. 
Cimponeriu et al. reported two cases of colonic histoplasmosis presenting as cancer. Case number one presented with cough, breathlessness, fever, weight loss and diarrhoea. The physical examination was unremarkable. The lesion mimicking cancer was found in right colon. Case number two presented with abdominal pain, fever, diarrhoea, anorexia and weight loss. On examination, cervical lymphadenopathy and hepatomegaly was noted. The lesion mimicking cancer was found in hepatic flexure. Both the cases were males and both were HIV seropositive. 
The case presented here had many unusual features. There was no prior clinical suspicion of HIV infection. There was involvement of only sigmoid colon and there was no associated hepatosplenomegaly, lymphadenopathy or oropharyngeal ulcer. Although colonic histoplasmosis mimicking carcinoma has been reported from various parts of the world, , this is first such report from India.
In cases of disseminated histoplasmosis, the yield of blood and sputum culture has been reported to be 75% and 50%, respectively.  However, we could not isolate H. capsulatum either from blood or sputum.
Due to varied and non-specific clinical manifestations of systemic histoplasmosis and low index of suspicion, most of the infections are either misdiagnosed or are under reported. With the continuing pandemic of AIDS and an ever-increasing pool of immunocompromised patients, it would not be wrong to predict an increase in incidence of histoplasmosis in the near future. It is therefore suggested that clinicians, microbiologists and pathologists be more aware of the clinical manifestations, risk factors and laboratory diagnosis of histoplasmosis.
|1||Kauffman CA. Histoplasmosis: A clinical and laboratory update. Clin Microbiol Rev 2007;20:115-32.|
|2||Cimponeriu D, LoPresti P, Lavelanet M, Roistacher K, Remigio P, Marfatia S, et al . Gastrointestinal histoplasmosis in HIV infection: Two cases of colonic pseudocancer and review of literature. Am J Gastroenterol 1994;89:129-31.|
|3||Subramanian S, Abraham OC, Rupali P, Zachariah A, Mathews MS, Mathai D. Disseminated histoplasmosis. J Assoc Physicians India 2005;53:185-9.|
|4||Goswami RP, Pramanik N, Banerjee D, Raza MM, Guha SK, Maiti PK. Histoplasmosis in eastern India: The tip of the iceberg? Trans R Soc Trop Med Hyg 1999;93:540-2.|
|5||McKinsey DS, Gupta MR, Riddler SA, Driks MR, Smith DL, Kurtin PJ. Long-term amphotericin B therapy for disseminated histoplasmosis in patients with the acquired immunodeficiency syndrome (AIDS). Ann Intern Med 1989;111:655-9.|
|6||McKinsey DS, Spiegel RA, Hutwagner L, Stanford J, Driks MR, Brewer J, et al . Prospective Study of histoplasmosis in patients infected with human immunodeficiency virus: Incidence, risk factors and pathophysiology. Clin Infect Dis 1997;24:1195-203.|
|7||Lee JT, Dixon MR, Murrell Z, Konyalian V, Agbunag R, Rostami S, et al . Colonic histoplasmosis presenting as colon cancer in the nonimmunocompromised patient: Report of a case and review of literature. Am Surg 2004;70:959-63.|
|8||Wheat J. Histoplasma, Chapter 278. In : Infectious Diseases, 2 nd ed. Gorbach SL, Bartlett JG, Blacklow NR, editors. W.B. Saunders Company: Philadelphia; 1998. p. 2339.|