Indian Journal of Medical Microbiology Home 

CORRESPONDENCE
[Download PDF]
Year : 2007  |  Volume : 25  |  Issue : 2  |  Page : 177--178

Circulating phage type of Vibrio cholerae in Mumbai

SD Turbadkar1, DP Ghadge2, S Patil3, AS Chowdhary1, R Bharadwaj1,  
1 Department of Microbiology, Grant Medical College, Sir JJ Group of Hospitals, Mumbai - 4000 008, Maharashtra, India
2 Department of Microbiology, BJ Medical College, Sassoon General Hospital, Pune - 411 001, Maharashtra, India
3 Department of Microbiology, Government Medical College, Sassoon General Hospital, Pune - 411 001, Maharashtra, India

Correspondence Address:
S D Turbadkar
Department of Microbiology, Grant Medical College, Sir JJ Group of Hospitals, Mumbai - 4000 008, Maharashtra
India

How to cite this article:
Turbadkar S D, Ghadge D P, Patil S, Chowdhary A S, Bharadwaj R. Circulating phage type of Vibrio cholerae in Mumbai.Indian J Med Microbiol 2007;25:177-178

How to cite this URL:
Turbadkar S D, Ghadge D P, Patil S, Chowdhary A S, Bharadwaj R. Circulating phage type of Vibrio cholerae in Mumbai. Indian J Med Microbiol [serial online] 2007 [cited 2019 Dec 16 ];25:177-178
Available from: http://www.ijmm.org/text.asp?2007/25/2/177/32738

Full Text

Dear Editor,

Cholera is endemic in several states of India. Bacteriophage typing is widely accepted as a convenient and highly discriminatory method of identifying epidemic strains of Vibrio cholerae . It is important to monitor the prevalent phage types within an area as introduction of new phage type may herald the onset of an outbreak.

A total of 200 faecal samples, received between January and December 2004 from patients with acute diarrhoea, were screened for the presence of V. cholerae by standard laboratory methods, [1] and identified by routine biochemical tests and confirmed by slide agglutination with antisera obtained from National Institute of Cholera and Enteric Diseases, Kolkata. Antibiotic susceptibility test was done by Kirby Bauer disc diffusion method. [2] The strains were then sent for phage typing to National Institute of Cholera and Enteric Diseases, Kolkatta. A total of 41 (20.5%) V.cholerae were isolated. All the strains were biotype El Tor, serotype Ogawa and belonged to phage type 4. These strains were typable with new scheme and were clustered in to type 27 (97.5%) and type 23 (2.4%) respectively. Vibrio cholerae isolates showed maximum sensitivity in vitro to gentamicin (92.6%) and chloramphenicol (87.8%) respectively [Table 1].

In spite of the complexities of the environment and a large migratory population in Mumbai, all the isolates belonged to Basu and Mukerjee phage type 4. Type 2 and 4 are the prevalent biotypes in India at present [3] but consistently getting only a single biotype over the year was surprising. However, the new scheme was more discriminatory and could identify two circulating types. Even then the majority belonged to types 27 (97.5 %) and only 2.4 % were type 23. These were found in the month of August and were likely to be imported phage type into the city.

Though prevalence of multi-drug resistant Vibrio cholerae has been reported from parts of India, [4],[5] we did not encounter any such strains. A decrease in sensitivity to ampicillin and tetracycline was observed.

References

1Cruickshank R, Duguid JP, Marimion BP, Swann RH. Medical Microbilogy. Vol. II, 12 th ed. Churchill Livingstone: Edinburgh; 1985. p. 440-2.
2Bauer AW, Kirby WM, Sherris JC, Turck MC. Antibiotic susceptibility testing by standardized single disc method. Am J Clin Pathol 1966; 45 :493-6 .
3Sundaram SP, Revathi J, Sarkar BL, Bhattacharya SK. Bacteriological profile of cholera in Tamil Nadu (1980-2001). Indian J Med Res 2002; 116 :258-63.
4 Taneja N, Mohan B, Khurana S, Sharma M. Antimicrobial resistance in selected bacterial enteropathogens in north India. Indian J Med Res 2004; 120 :39-43.
5 Mathur M, De A, Saraswathi K, Varaiya A, Athalye S. Vibrionaceae from cases of acute diarrhoea and their antimicrobial sensitivity pattern - A five year prospective study. Indian J Med Microbiol 2003; 21 :199-201.