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Year : 2003  |  Volume : 21  |  Issue : 4  |  Page : 265--267

Treatment of discarded blood units: Disinfection with hypochlorite / formalin versus steam sterilization

V Chitnis1, S Chitnis1, S Patil2, D Chitnis1 
1 Department of Microbiology, Choithram Hospital and Research Centre, Indore - 452 001, India
2 Department of Microbiology, School of Life Sciences, Devi Ahilya Vishwavidyalaya, Indore - 452 001, India

Correspondence Address:
D Chitnis
Department of Microbiology, Choithram Hospital and Research Centre, Indore - 452 001
India

Blood bank regulations and bio medical waste rules of India advocate disinfection of contaminated blood units. Incineration is not recommended due to poly-vinyl chloride (PVC) content of blood bags. This study was designed to evaluate the efficacy of chemical disinfection of blood units deliberately contaminated with Staphylococcus aureus and E. coli with 1 and 6 % hypochlorite, 10% formalin and 33% formaldehyde and autoclaving of blood units contaminated with the above mentioned vegetative forms and B. stearothermophilus spores. Only 33 % formaldehyde could bring about 5 log reduction of bacteria but it is highly irritating and toxic. Autoclaving at 15 lbs pressure for 2 hours uniformly inactivated the vegetative forms and B. stearothermophilus spores. Thus, autoclaving of PVC blood bags is a safer and reliable method compared to chemical disinfection.

How to cite this article:
Chitnis V, Chitnis S, Patil S, Chitnis D. Treatment of discarded blood units: Disinfection with hypochlorite / formalin versus steam sterilization.Indian J Med Microbiol 2003;21:265-267

How to cite this URL:
Chitnis V, Chitnis S, Patil S, Chitnis D. Treatment of discarded blood units: Disinfection with hypochlorite / formalin versus steam sterilization. Indian J Med Microbiol [serial online] 2003 [cited 2019 Dec 10 ];21:265-267
Available from: http://www.ijmm.org/article.asp?issn=0255-0857;year=2003;volume=21;issue=4;spage=265;epage=267;aulast=Chitnis;type=0