Indian Journal of Medical Microbiology Home 

Year : 2001  |  Volume : 19  |  Issue : 3  |  Page : 155--156

Penicillin resistant neisseria gonorrhoeae at Aurangabad

GS Bhatambare1, RP Karyakarte2,  
1 Microbiology Department, Govt. Medical College, Nanded, India
2 Microbiology Department, Govt. Medical College, Aurangabad, (RPK), Maharashtra, India

Correspondence Address:
G S Bhatambare
Microbiology Department, Govt. Medical College, Nanded


A total of 101 male patients with signs and symptoms suggestive of gonococcal urethritis were studied and 60 showed growth of Neisseria gonorrhoeae on culture. All the isolates were tested for antimicrobial susceptibility. Four strains of Neisseria gonorrhoeae were resistant to penicillin. The resistant strains were tested for production of b-lactamase. The rapid iodometric method for detection of b-lactamase showed that two strains produced b-lactamase.

How to cite this article:
Bhatambare G S, Karyakarte R P. Penicillin resistant neisseria gonorrhoeae at Aurangabad.Indian J Med Microbiol 2001;19:155-156

How to cite this URL:
Bhatambare G S, Karyakarte R P. Penicillin resistant neisseria gonorrhoeae at Aurangabad. Indian J Med Microbiol [serial online] 2001 [cited 2020 Sep 30 ];19:155-156
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Full Text

Gonorrhoea, a disease well documented from ancient times, continues to defy man's attempt to control it. The incidence of sexually transmitted disease is continuously increasing. Gonococcal infections and their complications are amongst the most frequent communicable diseases in many countries.[1] The present study was planned in view of increasing incidence and development of antibiotic resistance by Neisseria gonorrhoeae.

 Materials and Methods

Sixty strains of Neisseria gonorrhoeae were isolated from 101 male patients with suspected gonococcal urethritis from January 1996 to December 1997, in Microbiology department of Government Medical College, Aurangabad.

Pus and urine samples were collected by standard procedure.[2] These were first examined by Gram stain[3] and then inoculated on Modified Thayer-Martin medium and Chocolate agar,[3] and incubated at 37 C in candle jar for 18 to 24 hours. The growth was recorded. Gram staining,[3] oxidase test,[3] sugar degradation test[4] and antimicrobial sensitivity tests[1],[5] were performed.

The antimicrobial susceptibility test[1],[5] using Kirby-Bauer disk diffusion method was carried out with ampicillin 10 g, cefotaxime 30 g, ciprofloxacin 3 g, co-trimoxazole 15 g, kanamycin 30 g, penicillin G 10 units and tetracycline 30 g.

The penicillin resistant isolates of Neisseria gonorrhoeae were tested for the production of b-lactamase. Rapid iodometric test was used for the detection of b-lactamase.[4]


The four strains of Neisseria gonorrhoeae resistant to penicillin were tested for the production of b-lactamase. The rapid iodometric method for detection of beta-lactamase showed that two out of four strains produced b-lactamase. [Table:1] shows results of antimicrobial susceptibility testing.


The rapid iodometric method was performed on four penicillin resistant strains of Neisseria gonorrhoeae and it was found that two of them produced b-lactamase. This is an alarming sign as, there were no strains of Neisseria gonorrhoeae reported to be resistant to penicillin between1986 and 1987 in Aurangabad.6 Within ten years the incidence of penicillin resistance in Neisseria gonorrhoeae has risen to 6.66 % at Aurangabad. This emphasizes the importance of antimicrobial susceptibility testing in the course of treatment of gonorrhoea.

The b-lactamase producing Neisseria gonorrhoeae were detected for the first time in Aurangabad during this study. b-lactamase producing strains have been reported earlier in India from Madras (Chennai),[7] Bombay (Mumbai),[8] Trivandrum (Thiruvananthapuram),[9] Vishakhapatnam[10] and Chandigarh.[11]

[Table:2] shows the results of studies carried out in India to detect b-lactamase producing strains of Neisseria gonorrhoeae


We thank the Dean, Government Medical College, Aurangabad, for providing us necessary facilities.


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