Indian Journal of Medical Microbiology Home 

Year : 1999  |  Volume : 17  |  Issue : 4  |  Page : 190--193

Dot ELISA for antigen specific Circulating immune complex detection in Kala-azar

J Amita, B Mamta 
 Department of Microbiology, King George's Medical College, Lucknow 226 003, U.P, India

Correspondence Address:
J Amita
Department of Microbiology, King George«SQ»s Medical College, Lucknow 226 003, U.P
India

ABSTRACT: Present study compares two simple ELISA tests for detection of antigen specific immune complexes (CIC) in the sera of patients of visceral leishmaniasis (VL). Their role in diagnosis of VL is evaluated. Sera from 50 positive controls, 50 negative controls (25 each endemic and nonendemic) and 40 patients (10 each of leprosy, malaria, amoebic hepatitis and tuberculosis) were tested. Immune complexes were precipitated with polyethylene glycol and their presence was tested by microwell and dot-ELISA. It was noted that both the ELISA were 100 percent sensitive, while dot ELISA was more specific compared to microwell ELISA (97.7 percent and 91.1 percent respectively with dot and microwell ELISA). The cross reactivity with dot-ELISA was 5.0 percent and with microwell ELISA, 20.0 percent. Hence dot-ELISA is preferred over microwell ELISA because it is simple to perform, needs less test time, is adaptable for field testing, needs least laboratory facilities and is more specific.

How to cite this article:
Amita J, Mamta B. Dot ELISA for antigen specific Circulating immune complex detection in Kala-azar.Indian J Med Microbiol 1999;17:190-193

How to cite this URL:
Amita J, Mamta B. Dot ELISA for antigen specific Circulating immune complex detection in Kala-azar. Indian J Med Microbiol [serial online] 1999 [cited 2019 Sep 15 ];17:190-193
Available from: http://www.ijmm.org/article.asp?issn=0255-0857;year=1999;volume=17;issue=4;spage=190;epage=193;aulast=Amita;type=0