Indian Journal of Medical Microbiology Home 

Year : 1995  |  Volume : 13  |  Issue : 3  |  Page : 145--149

comparative studies on the susceptibility of certain primary cultures to coxiella burnetii

SS Kulkarni, RR Blonde, VS Ladybird 
 National Institute of Virology, 20-A, Dr, Ambedkar Road, Pune 411 001

Correspondence Address:
SS Kulkarni
National Institute of Virology, 20-A, Dr, Ambedkar Road, Pune 411 001

ABSTRACT: Ten primary cultures were screened to determine their susceptibility to Coxiella burnetii, the causative organism of Q fever. The most susceptible culture could then the be employed as an alternative system to the conventional yolk sac method for the efficient cultivation of these organisms. The primary cultures examined consisted of four each, derived from chick and mouse and two of human origin. The criteria followed for determining the susceptibility were microscopic counting of the number of infected cells, antigenicity and egg lethality assay. The maximum multiplication of C.burnetii was observed in the two human foetal cultures-human embryonic lung and kidney with cytopathic effect in the form of vacuolated cytoplasm. In the remaining cultures there was mild to moderate growth of C.burnetii. Our studies have generated sufficient data to show that, for the first time, certain primary cultures like human embryonic kidney, mouse embryonic brain, chorio-allantoic membrane and mouse bone marrow, are being reported as being susceptible to C.burnetii.

How to cite this article:
Kulkarni S, Blonde R, Ladybird V. comparative studies on the susceptibility of certain primary cultures to coxiella burnetii.Indian J Med Microbiol 1995;13:145-149

How to cite this URL:
Kulkarni S, Blonde R, Ladybird V. comparative studies on the susceptibility of certain primary cultures to coxiella burnetii. Indian J Med Microbiol [serial online] 1995 [cited 2020 Jul 2 ];13:145-149
Available from: http://www.ijmm.org/article.asp?issn=0255-0857;year=1995;volume=13;issue=3;spage=145;epage=149;aulast=Kulkarni;type=0