Indian Journal of Medical Microbiology Home 

Year : 1995  |  Volume : 13  |  Issue : 3  |  Page : 115--118

Protein and isoenzyme patterns of mycobacteria I. their role in identification of rapidly growing mycobacteria

VD Sharma, VM Katoch, CT Shivannavar, UD Gupta, RK Sharma, MS Patil, VP Bharadwaj, BM Agrawal 
 Dept of Microbiology, Central JALMA Institute for leprosy (ICMR), Taj Ganj, Agra

Correspondence Address:
VD Sharma
Dept of Microbiology, Central JALMA Institute for leprosy (ICMR), Taj Ganj, Agra

ABSTRACT: Cell free extracts (CFEs) from different rapidly growing mycobacteria namely, M.fortuitum, M.chelonei, M. vaccae, M.smegmatis, M.phlei and M.duvalii were electrophoresed and stained for proteins as well as lactate dehydrogenase (LDH), esterase, malate dehydrogenase (MDH), malic enzyme (ME), alanine dehydrogenase (Alanine DH), superoxide dismutase (SOD), alcohol dehydrogenase (Alcohol DH) and succinic dehydrogenase (SDH) activity. It was observed that combination of the protein, LDH, esterase and ME produced clearly distinguishable patterns for all the mycobacteria included in the study. Results described in the paper establish a simple electrophoretic technique for rapid identification of fast growing mycobacteria.

How to cite this article:
Sharma V, Katoch V, Shivannavar C, Gupta U, Sharma R, Patil M, Bharadwaj V, Agrawal B. Protein and isoenzyme patterns of mycobacteria I. their role in identification of rapidly growing mycobacteria.Indian J Med Microbiol 1995;13:115-118

How to cite this URL:
Sharma V, Katoch V, Shivannavar C, Gupta U, Sharma R, Patil M, Bharadwaj V, Agrawal B. Protein and isoenzyme patterns of mycobacteria I. their role in identification of rapidly growing mycobacteria. Indian J Med Microbiol [serial online] 1995 [cited 2020 Jul 5 ];13:115-118
Available from: http://www.ijmm.org/article.asp?issn=0255-0857;year=1995;volume=13;issue=3;spage=115;epage=118;aulast=Sharma;type=0