Indian Journal of Medical Microbiology Home 

Year : 1991  |  Volume : 9  |  Issue : 4  |  Page : 193--201

Heat Labile Enterotoxin By Various Salmonellae

Manish Pandya1, Parukutty K Pillai2, Shashi Khare3, Manorama Deb4, Sudarshan Kumari5, Kunti Prakash6 
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Correspondence Address:
Manish Pandya


93 strains of Salmonella belonging to 5 different serovars namely S.typhimurium, S.senftenberg, S.bareilly, S.welteverden and S.newport isolated from fecal samples of patients suffering from gastroenteritis were studied for the production of enterotoxin. The enterotoxin production was detected by using CHO, Vero and HeLa cell lines. Out of 93 strains tested enterotoxin production was shown by 63 strains (67.74%) in CHO cell assay, 56 strains (60.21%) in Viro cell assay and 31 strains (33.33%) in HeLa cell assay. The enterotoxin was found to be heat labile as it lost all its activity on cell lines on heating at 60 degree centigrade for 30 minutes and at 100 degree centigrade for 15 minutes. The effect of Salmonella heat labile enterotoxin (S-LT) on CHO, Vero and HeLa cell lines was found to be reversible. S-LT was neutralized by monoclonal anticholera enterotoxin. CHO cell assay was found to be a more sensitive assay for detection enterotoxigenicity of 6 additional strain of S-typhimurium and 1 additional strain of S-bareilly which was not detected in other two cell lines.

How to cite this article:
Pandya M, Pillai P, Khare S, Deb M, Kumari S, Prakash K. Heat Labile Enterotoxin By Various Salmonellae.Indian J Med Microbiol 1991;9:193-201

How to cite this URL:
Pandya M, Pillai P, Khare S, Deb M, Kumari S, Prakash K. Heat Labile Enterotoxin By Various Salmonellae. Indian J Med Microbiol [serial online] 1991 [cited 2020 Sep 25 ];9:193-201
Available from: http://www.ijmm.org/article.asp?issn=0255-0857;year=1991;volume=9;issue=4;spage=193;epage=201;aulast=Pandya;type=0