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 BRIEF COMMUNICATION
Year : 2019  |  Volume : 37  |  Issue : 1  |  Page : 105-108

Use of TaqMan Array card for the detection of respiratory viral pathogens in children under 5 years old hospitalised with acute medical illness in Ballabgarh, Haryana, India


1 Department of Microbiology, All Institute of Medical Sciences, New Delhi, India
2 Influenza Division, Centers for Disease Control and Prevention, U.S Embassy, New Delhi, India
3 Influenza Division, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
4 Centre for Community Medicine, All Institute of Medical Sciences, New Delhi, India
5 Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
6 Division of Bacterial Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA

Correspondence Address:
Dr. Bharti Gaur
Centre for Community Medicine, All India Institute of Medical Sciences, New Delhi - 110 029
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/ijmm.IJMM_18_146

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Historical specimens collected from hospitalized children were tested for the following 13 viruses: influenza A and B; respiratory syncytial virus (RSV); parainfluenza viruses 1–3; human metapneumovirus; rhinovirus; coronaviruses 229E, OC43, NL63 and HKU1 and Adenovirus using monoplex real-time reverse transcriptase polymerase chain reaction (rRT-PCR). They were retested using TaqMan Array Card (TAC), a micro-fluidic system, capable of simultaneous multi-pathogen testing, to evaluate its sensitivity and specificity against monoplex rRT-PCR. TAC showed high sensitivity (71%–100%) and specificity (98%–100%) for these viruses in comparison to monoplex rRT-PCR. Multi-specimen detection with high sensitivity and specificity makes TAC a potentially useful tool for both surveillance and outbreak investigations.






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2004 - Indian Journal of Medical Microbiology
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