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Year : 2018  |  Volume : 36  |  Issue : 3  |  Page : 429-431

Serotyping of dengue viruses circulating during 2014–2015 in Assam, India


1 Department of Microbiology, Dr. Damanis Nursing Home, Dibrugarh, Assam, India
2 Department of Microbiology, Gauhati Medical College, Guwahati, Assam, India
3 Regional Medical Research Centre, Dibrugarh, Assam, India
4 Department of Microbiology, Assam Medical College, Dibrugarh, Assam, India

Correspondence Address:
Dr. Ajanta Sharma
Department of Microbiology, Gauhati Medical College, Guwahati - 781 032, Assam
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/ijmm.IJMM_17_121

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Dengue has become endemic in India with outbreaks caused by all the four serotypes occurring almost every year. Dengue cases have been increasing alarmingly in Assam also. This study aimed to identify the dengue serotypes circulating in Assam. Clinically suspected dengue fever cases were included in the study. Viral RNA was extracted using QIAamp Viral RNA Mini Kit (Qiagen). Nested reverse transcriptase polymerase chain reaction was done for serotyping. The frequency of dengue was 25.23% with a peak during the period from September (22.56%) to October (26.22%). Two serotypes were detected, DEN-1 (72.7%) and DEN-2 (12.1%) and dual infection in 15.1%.






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