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 ORIGINAL ARTICLE
Year : 2018  |  Volume : 36  |  Issue : 1  |  Page : 81-86

Identification of multiple strains of Porphyromonas gingivalis using heteroduplex polymerase chain reaction in varying severity of chronic periodontitis


1 Department of Periodontics, SDM College of Dental Sciences and Hospital, Dharwad, Karnataka, India
2 Department of Microbiology, Maratha Mandal Dental College and Hospital, Belgaum, Karnataka, India
3 Department of Periodontology, Faculty of Dentistry, Mahsa University, 59100 Kuala Lumpur, Malaysia
4 Department of Periodontology, Manipal College of Dental Sciences, Manipal, Karnataka, India
5 Department of Microbiology, SDM College of Medical Sciences and Hospital, Dharwad, Karnataka, India

Correspondence Address:
Dr. Raghavendra Dhirendra Kulkarni
Department of Microbiology, SDM College of Medical Sciences and Hospital, Dharwad - 580 009, Karnataka
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/ijmm.IJMM_17_434

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Aim: Research has demonstrated that there are multiple strains of Porphyromonas gingivalis with varying potency to cause periodontal disease. The current study aims at using heteroduplex polymerase chain reaction (PCR) to detect the strain diversity of P. gingivalis in periodontitis lesions of varying severity in a sample of the Indian population. Materials and Methods: Subgingival plaque samples were collected from 60 individuals with varying severity of chronic periodontitis and 30 individuals with a clinically healthy periodontium. The samples were subjected to PCR analysis to identify P. gingivalis, followed by heteroduplex analysis to identify the strain diversity in a given sample. Bacterial culture was carried out as a comparative standard. Results: Of the 56 samples that were positive for P. gingivalis by PCR, 54 samples yielded eight different heteroduplex patterns. Analysis of these patterns indicated that two strains of P. gingivalis were present in 41 individuals (45.6%) and three strains were present in 13 individuals (14.4%). Detection of P. gingivalis by PCR was significantly more in the periodontitis group as compared to the healthy group. Conclusions: Species-specific PCR and heteroduplex analysis provide a simple and accurate method to analyse the strain diversity of P. gingivalis. P. gingivalis was detected in both healthy periodontal sites as well as sites with periodontitis. The presence of two or three P. gingivalis strains was seen in 60% of the samples.






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2004 - Indian Journal of Medical Microbiology
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