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 BRIEF COMMUNICATION
Year : 2017  |  Volume : 35  |  Issue : 4  |  Page : 588-592

Clinically relevant yeast species identified by sequencing the internal transcribed spacer region of r-RNA gene and Vitek 2 compact (YST card) commercial identification system: Experience in a Tertiary Care Hospital in Assam, Northeast India


1 Department of Microbiology, Assam Medical College and Hospital, Dibrugarh, Assam, India
2 Department of Paediatrics, Assam Medical College and Hospital, Dibrugarh, Assam, India
3 Regional Medical Research Center, Dibrugarh, Assam, India

Correspondence Address:
Dr. Reema Nath
Department of Microbiology, Assam Medical College and Hospital, Dibrugarh - 786 002, Assam
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/ijmm.IJMM_17_100

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In this retrospective study from 2012 to 2015, 333 clinical isolates of yeasts were identified using Vitek 2 Compact System YST ID card (Biomerieux, France) and internal transcribed spacer (ITS) sequencing. Eighteen species were identified by ITS sequencing. Candida albicans was the most common species (46.5%), followed by Candida tropicalis (27%). The total species supported by Vitek System was 11 (61.11%). The sensitivity of the system in identifying these 11 species was 66.66%–100%; specificity 98.37%–100%; positive predictive value 70%–100%, negative predictive value 96.05%–100%, and diagnostic accuracy 96.99%–100%. Diagnostic accuracy of ITS1 and ITS2 sequences individually was 98.49% and 100% using NCBI Genbank database.






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